Timed dose-response relationship of pain in autistic model rats

doi:10.12092/j.issn.1009-2501.2019.12.004

Abstract

Abstract:

AIM: To observe the characteristics of timed dose-response relationship (TDRR) of pain in autistic model rats. METHODS: Ten pregnant SD rats were randomly divided into model pregnant group (n=6) and normal pregnant group(n=4). The model pregnant group rats were prepared by intraperitoneal injection of sodium valproate 600 mg/kg at 12.5 days after pregnancy and equivalent volume of saline was used as control injection in normal pregnant group.Then their offspring were used as model group (n=24) and normal group (n=22),respectively.During the period of 35 days after birth, a series of behavior tests were measured to identify the autistic model rats. Thereafter, the paw withdrawal mechanical threshold (PWMT) and tail flick latency (TFL) evoked by light radiant heat were measured from these rats in two groups.RESULTS:The growth and development indicators and the ability of behavioral exploration in model group rats were both less than those in normal group (P<0.01).Compared with normal group rats,the PWMT of the model group rats increased significantly (P<0.01).TFLs of rats in two groups were both shortened with increased light intensities, indicating a hyperbolic type-like TDRR. However, the TFLs in the TDRR curve of model group rats were longer than those of normal group rats(P<0.01). CONCLUSION: These preliminary results suggest that the autistic model rats increase the threshold of mechanical and heat pain, and change the TDRR curve of TFL to right either.

Key words: autism, pain, timed dose-response relationship

CLC Number:

R965.2

YANG Liqin, LI Zhiyu, TAN Pei, XU Wenting, ZHANG Zhongnan, WANG Mengya. Timed dose-response relationship of pain in autistic model rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1341-1346.

References

［1］段云峰,吴晓丽,金锋.自闭症的病因和治疗方法研究进展［J］.中国科学:生命科学,2015,45(9):820-844.
［2］刘智敏，张亚兰，张春莉，等.认知-行为干预对自闭症儿童的影响分析［J］.心理医生,2016,22(9):93-94.
［3］Klintwall L,Holm A,Eriksson M, et al.Sensory abnormalities in autism. A briefreport［J］.Res Dev Disabi,2011,32(2):795-800.
［4］Elwin M, Ek L,Schroder A,et al.Autobiographical accounts of sensing in asperger syndrome and high functioning autism［J］.Arch Psychiat Nurs,2012,26(5):420-429.
［5］金雪莲，李少聪.自闭症谱系障碍儿童认知特点［J］.吉林医药学院学报，2019，40(1)：43-45.
［6］Dubois A,Rattaz C,Pry R,et al.Autism and pain-a literature review［J］.Pain Res Manag,2010,15(4):245-253.
［7］Cravero C,Guinchat V,Barete S,et al.Cornelia de Lange and Ehlers-Danlos:comorbidity of two rare syndromes［J］.BMJ Case Rep,2016,2(1):1-4.
［8］汪萌芽,孙瑞元.时反应量效关系及其研究现状［J］.中国药理学通报,1987,3(2):111-114.
［9］汪萌芽，孙瑞元.时反应量效关系数学模型的探讨［J］.科技通报,1986,2(4):33-36.
［10］余雪霏,张铭勋,方博文，等.大鼠光辐射热甩尾试验的时反应量-效关系［J］.皖南医学院学报,2014,33(2):176-180.
［11］Schneider T,Przewocki R.Behavioral alterations in rats prenatally exposed to valproic acid:animal model of autism［J］.Neuropsychopharmacol,2005,30(1):80-89.
［12］崔利军,郭岚敏,郭津，等.丙戊酸钠诱导孤独症大鼠模型复制的实验研究［J］.中国中西医结合儿科学,2013,5(2):122-125.
［13］祁梦.利培酮对孤独症大鼠学习记忆功能的影响［D］.皖南医学院,2016.
［14］王软娜.VPA诱导小鼠自闭症模型中小脑功能及发育障碍研究［D］.广州：暨南大学,2018.
［15］傅萍.针刺“长强”穴对自闭症模型大鼠海马区NF-KB、Bcl-xL蛋白表达的影响［D］.福建：福建中医药大学，2018.
［16］刘海鹰，范运龙，曹阳，等.不同因素对Shank3B~(-/-)孤独症模型小鼠刻板行为分析的影响［J］.神经解剖学杂志，2018，34(1)：21-26.
［17］常永丽，许银红，温雪敏，等.地佐辛复合酮咯酸氨丁三醇超前镇痛对大鼠术后痛阈和炎性反应的影响［J］.中国临床药理学与治疗学，2017，22(3)：267-271.
［18］雷雨溪,何慕兰,赖茜,等.两种孤独症谱系障碍大鼠模型的构建及比较［J］.重庆医科大学学报,2018,43(09):1132-1139.
［19］Stromland K, Nordin V,Miller M,et al.Autism in thalidomide embryopathy: a population study［J］.Dev Med Child Neurol,2010,36(4):351-356.
［20］李晋,宋天佳,孟凡超，等.孤独症与正常儿童的痛阈比较研究［J］.中国疼痛医学杂志,2015,21(12):908-913.
［21］郑青山.药效动力学数学模型及其评价［J］.中国临床药理学与治疗学杂志,1997，2(4):283-286.
［22］Chirag M,Annesha S,Tammy H,et al.Childhood disintegrative disorder and autism spectrum disorder: a systematic review［J］.Dev Med Child Neurol,2019,61(5):523-534.
［23］高会军，王丽平，曹清花.75例自闭症患儿应用多感官互动治疗的效果分析［J］.临床研究,2019,27(3):63-64.
［24］邹小兵.孤独症谱系障碍干预原则与BSR模式［J］.中国儿童保健杂志,2019,27(1):1-6.

[1]	ZHAO Lu, LI Fuyong, WEN Binhong, LI Daowei. Effects of stains on hyperlipidemia and pain relieve of patients with chronic migraine [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 671-675.
[2]	ZHANG Qiang, ZHENG Huajun, LI Quan. Research progress on the treatment of autism spectrum disorders based on gut microbiota intervention [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(4): 475-480.
[3]	Qin LiYang. Effects of rapamycin intervention during pregnancy on cognitive function of autism model in rat offspring [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 841-847.
[4]	JIA Tao, TENG Jinliang. New narcotic analgesics: esketamine [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(7): 834-840.
[5]	SHEN Yanping, YIN Lijun, ZHUANG Wenming, YAN Haiya. Effective dose of esketamine for prevention on propofol injection pain in painless abortion [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 660-664.
[6]	ZHOU Haihui, ZHANG Haixia, GE Weihong. Analysis of the role of clinical pharmacist in the establishment of standardized cancer pain management ward [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(9): 1038-1042.
[7]	HU Peipei, HUANG Fang. Yunnan Baiyao ameliorates MIA-induced knee osteoarthritis pain in rats through anti-inflammatory effect [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(3): 254-259.
[8]	FAN Yimin, WANG Limin, GUO Xiaoshu, FENG Gaizhuang, LI Xiuping. Lumbar plexus block with Ropivacaine for hind limbs pain of chronic ischemia in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 400-405.
[9]	XU Xiangwei, CHEN Yanqiao, ZHU Peizhen. Therapeutic effect of amtiriptyline combined with opioids for moderate to severe cancer pain patients with depression [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(10): 1145-1151.
[10]	FEI Xianming, WANG Huan, YUAN Wufeng, JIANG Lei, CHENG Maoliang. Effects and preliminary molecular mechanisms of papain on the inhibition of monocyte secretory and adherent ability induced by monocyte-platelet aggregates [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 1-8.
[11]	FU Shuang, ZHOU Hui-dan, YUAN Xiao-hong, QU Pi-sheng. Effect of perioperative application of gabapentin on chronic pain of post-thoracotomy [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 583-586.
[12]	JIANG Lei, FEI Xian-ming, MIAO Cheng-lin, YUAN Wu-feng, QIU Lian-nv. Effects of papain on monocyte-platelet aggregates formation and monocyte activation induced by monocyte-platelet aggregates [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 289-292.
[13]	WANG Li-feng, ZHANG Zhi-yu, LI Zhen-zhen, LIANG Ying-xia. Research progress of therapy of intravenous immunoglobulin on neuropathic pain [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(2): 213-216.
[14]	HUANG Yang, TANG Ying, LIU Bin, BAO Cheng-jia, LIN Chun. Inhibitory effect of ZD7288 on visceral hypersensitivity in rats with acute visceral pain [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 721-726.
[15]	GONG Deng-hui, ZHENG Wei-hong, LUO Ni, TANG Gui-chen. Effects and the mechanism of gastrodin on chemotherapy-induced neuropathic pain through the expression of Iba1 of spinal dorsal horn [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 743-746.