Abstract: AIM: To investigate the expression of NF-κB and IκB in rats with adriamycin nephropathyand the intervention of Dahuangzhechong Wan.METHODS: The rats were injected with 5 mg/kg adriamycin solution from vena caudalis, then the rats were divided into two groups randomly: Adriamycin nephropathy group(n＝30) and Dahuangzhechong Wan(DHZCW) group(n＝15), and the other 8 rats were selected as the control group. On the 7th day, the 14th day, 4 rats from the model group were sacrificed, the renal tissue was reserved for detection. On the 28th day, the rats were all put to death.The changes of the renal tissue ultrastructure were observed. Immunofluorescence, immunohistochemistry and quantitive analysis were used to detect the expression of NF-κB and IκB-α.RESULTS: Compared with the control rats, the expression of NF-κB in model group increased markedly[(2.491±0.551)×10⁶], accompanying with the down-regulation of IκB-α[(0.947±0.631)×10⁶], and the podocyte was injuried. DHZCW could suppress the expression of NF-κB[(1.165±0.374)×10⁶ ], lessen the podocyte lesion.CONCLUSION: DHZCW has the protection effect on podocyte by influencing the expression of NF-κB and IκB.

Key words: Dahuangzhechong Wan, NF-κB, IκB, Adriamycin nephropathy