Oridonin up-regulated the expression on prostate cancer PC-3 cell PTEN gene and its apoptotic effect

Abstract

Abstract: AIM: To investigate apoptosis of human prostate cancer cell line PC-3 induced by oridonin and the expression relationship with PTEN gene. METHODS: The growth inhibition of PC-3 cell induced by oridonin was analyzed by MTT assay.The apoptosis of cell was detected by analyzing DNA ploid with flowcytomery.The mRNA expression of PTEN gene were detected by reverse transcription polymerase chain reaction (RT-PCR) assay. RESULTS: The proliferation effect of PC-3 cell was significantly inhibited induced by oridonin with dose-and time-dependent manners.The percentage of apoptosis in PC-3 cells was increased with a time-dependent induced by oridonin.With the increasing of apoptosis cell,the mRNA expression of PTEN gene was gradually enhanced. CONCLUSION: Oridonin obviously inhibits the proliferation of PC-3 cell and induces the apoptosis of prostate cancer,and its pathways of action maybe relate with up-regulated the expression of PTEN mRNA.

Key words: Oridonin, Prostate cancer, PTEN, Apoptosis

CLC Number:

R965.2

LU Zi-wen, WANG Chen, ZHANG Xian-jun, ZHU Ming, YU Hong-yuan, HUANG Li-fu, WANG Tian-ji. Oridonin up-regulated the expression on prostate cancer PC-3 cell PTEN gene and its apoptotic effect[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(10): 1105-1109.

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