Table of Content

Volume 15 Issue 10
26 October 2010

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Effects of benzodiazepine on amnestic effect induced by etomidate in mice

HUANG He, LI Bing, HAN Dan, MA Ke, ZHU Yang-zi, WU Yu-qing

2010, 15(10):  1092-1095. 

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AIM: To observe the effects of flumazenil (Flu) on amnestic effect induced by etomidate in mice. METHODS: 48 Kunming mice were randomly divided into 4 groups: NS+intralipid (Z) Group, NS+etomidate (Eto) Group, Flu+Z Group, Flu+Eto Group. All mice were given Flu(0.1 mg/kg)or NS(0.1 mL/10 g) by subcutaneous injection 20 min before training, then mice in all groups were administered intralipid (0.12 mL/10 g) or Eto (3 mg/kg) by intraperitoneal injection 5 min before training. All mice were then conducted to take step-down test and step-through test. The latency of step through and step down as well as the errortimes were observed to evaluated the effects of flumazenil on the amnestic effect induced by etomidate. RESULTS: Etomidate significantly increased error times and shortened the step down latency and step through latency of mouse. CONCLUSION: Benzodiazepines (BDZ) receptor may be an important target for the amnestic effect induced by etomidate.

Protective effects of lycopene on hepatic injury induced by tripterygium glycosides in mice

HE Jun, ZHAO Chun-jing, LIAO Chang-jun, YANG Zheng, TANG Ying

2010, 15(10):  1096-1099. 

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AIM: To explore the protective effect of lycopene on acute hepatic injury in mice caused by tripterygium glycosides. METHODS: 40 mice were divided into 4 groups randomly: control group,model group, lycopene high dose group(20 mg/kg) and lycopene low dose group(10 mg/kg).The rat models of hepatic injury were established by twentyfold(270 mg/kg) after 6 d prophylactic administration. ALT and AST activities in serum, SOD and MDA contents in liver and histopathological examination were detected to study the hepatoprotective effect of lycopene. RESULTS: Lycopene showed significant protection against the hepatotoxic actions of tripterygium glycosides,markedly alleviated AST and ALT levels in serum, enhanced SOD content,reduced MDA content in liver,and reduced hydropic degeneration. CONCLUSION: Lycopene has protective effects on hepatic injury induced by tripterygium glycosides in mice.

Comparing study on anti-inflammatory and analgesic effect and mechanism of Qizheng Qingpeng Paste and capsaicin cream

XU Wen-pin, WANG Xin, LI Min, LI Wei-dong

2010, 15(10):  1100-1104. 

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AIM: To investigate the similarity and difference of effects and mechanisms between Qingpeng Paste and capsaicin cream on arthritis. METHODS: The rats of formalin inflammatory pain model were pretreated with the two drugs, pathologic changes and substance P expression of the tissues, serum NO level and the plasma level of β endorphin were measured, and the behavior change was observed. RESULTS: Compared with model group, each dosage of Qingpeng Paste and capsaicin cream could greatly improve the pathologic changes and decrease the level of substance P. Two dosages of Qingpeng Paste and high dosage of capsaicin cream could inhibit the second phase of formalin inflammatory pain, decrease the level of serum NO, and increase the level of plasma β endorphin. Compared with capsaicin cream, Qingpeng Paste could restrain the fist phase of formalin inflammatory pain, had no simulative effect of high dosage capsaicin cream and had an analgesic effect in a dose-dependant manner. CONCLUSION: There is much comparability between effects and mechanism of Qingpeng Paste and capsaicin cream on arthritis. However, comparing with capsaicin, Qingpeng Paste might has wider and more controllable pharmacological functions.

Oridonin up-regulated the expression on prostate cancer PC-3 cell PTEN gene and its apoptotic effect

LU Zi-wen, WANG Chen, ZHANG Xian-jun, ZHU Ming, YU Hong-yuan, HUANG Li-fu, WANG Tian-ji

2010, 15(10):  1105-1109. 

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AIM: To investigate apoptosis of human prostate cancer cell line PC-3 induced by oridonin and the expression relationship with PTEN gene. METHODS: The growth inhibition of PC-3 cell induced by oridonin was analyzed by MTT assay.The apoptosis of cell was detected by analyzing DNA ploid with flowcytomery.The mRNA expression of PTEN gene were detected by reverse transcription polymerase chain reaction (RT-PCR) assay. RESULTS: The proliferation effect of PC-3 cell was significantly inhibited induced by oridonin with dose-and time-dependent manners.The percentage of apoptosis in PC-3 cells was increased with a time-dependent induced by oridonin.With the increasing of apoptosis cell,the mRNA expression of PTEN gene was gradually enhanced. CONCLUSION: Oridonin obviously inhibits the proliferation of PC-3 cell and induces the apoptosis of prostate cancer,and its pathways of action maybe relate with up-regulated the expression of PTEN mRNA.

Effects of metallothionein induced by ZnSO₄ on pneumocyte apoptosis after lung ischemia/reperfusion injury in rats

SHI Lu, JIA Xu-guang, QIU Xiao-xiao, QIAN Xiao-ying, WANG Yang, XU Dong-wu, WANG Wan-tie

2010, 15(10):  1110-1115. 

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AIM: To investigate the effects of metallothionein induced by ZnSO₄ on pneumocyte apoptosis after lung ischemia/reperfusion injury in rats. METHODS: Adult male Sprague-Dawley rats were randomly divided into 3 groups based upon the intervention (n=8): control group (C), LIR group (I/R), LIR+ ZnSO₄ group (M). At the end of the experiment, the content of metallothionein was tested in lung tissue; blood specimens drawn from the arteria carotis were tested for the content of Malondialdehyde (MDA), the activity of Superoxide dismutase (SOD) and Myeloperoxidase (MPO); the pneumocyte apoptosis index (AI) was achieved by terminal deoxynucleotidyl transferase mediated dUTP nick end abeling (TUNEL); the expression of Bcl-2, Bax protein in lung tissue were accessed by quantitative immunohistochemistry (IHC) and Bcl-2, Bax mRNA by RT-PCR; ultrastructural changes of lung tissue were ob- served by transmission electron microscope. RESULTS: Metallothionein induced by ZnSO₄ could significantly attenuate the MDA level, MPO activity and improve SOD activity in blood serum which was comparable to I/R and significantly reduced the number of TUNEL-positive cells vs. the I/R group, expressed as AI (% total nuclei) from 39.03±3.46 to 15.50±1.02 (P<0.01). The protein and mRNA expression of Bcl-2, Bax showed that metallothionein significantly attenuated the ischemia/ reperfusion-upregulated expression of Bax protein but improved the expression of Bcl-2 that improved the Bcl-2/ Bax ratio(P<0.05 or P <0.01). There were abnormal changes of the ultrastructure in I/R, and markedly reversed in MT group. CONCLUSION: Metallothionein induced by ZnSO₄ may attenuate pneumocyte apoptosis in LIRI by up-regulating expression of Bcl-2/ Bax ratio and by inhibiting oxidant generation, neutrophils filtration.

Mechanism of Apocynin improves insulin resistance induced by TNF-α in HepG2 cells

LI Lan-fang, YUO Yu, TANG Guo-tao, YU Cui-yun, CHEN Lin-xi

2010, 15(10):  1116-1121. 

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AIM: To investigate the effects of Apocynin on oxidative stress and insulin resistance induced by TNF-α. METHODS: Cells were divided into control group,TNF-α(4 ng/mL)treatment group,TNF-α+Apocynin(100 μmol/L)treatment group,Apocynin(100 μmol/L)group. The insulin resistance cell model was induced by TNF-α(4 ng/mL)to stimulate human hepatoma carcinoma cell HepG2. The intracellular glycogen was detected using a glucose oxidase assay kit. The level of intracellular reactive oxygen species (ROS) was detected by DCFH-DA fluorescent probe and flow cytometry. The expressions of JNK, p-JNK, IRS1 and p-IRS1 were observed by Western blotting. RESULTS: Compared with the control group, the level of ROS in HepG2 Cells in TNF-α treatment group was significantly increased and the level of intracellular glycogen in cell was decreased, TNF-α activated JNK and inhibited insulin signal pathway,and Apocynin could reverse those effects induced by TNF-α.Apocynin(100 μmol/L)group had no significant effects on ROS,glycogen synthesis and signal pathway. CONCLUSION: Apocynin can decrease the level of ROS in cell and improve insulin resistance condition induced by TNF-α in HepG2 cell.

Effects of soybean isoflavones on ethology and antioxidative potential of AD model rats

SUN Jing, WEI Rui-li, ZOU Ming, WANG Xiao-tong

2010, 15(10):  1122-1127. 

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AIM: To study the effects of different doses of soybean isoflavone (SIF) on ethology and antioxidative potential of AD model rats. METHODS: Sixty healthy male SD rats were randomly divided into sham operation group; model group; low, medium, high dose SIF groups and positive control group, respectively, with each group including 10 rats. The regulated dose medicine was given to the SIF groups and positive control group by intragastric administration daily for 14 days before Aβ injection, sham operation group and model group were given equivalent of 0.5% CMC-Na. Morris water maze experiment was adopted to evaluate the learning and memory ability of rats in each group on the 7th day after Aβ injection, then the pathological change in rats' hippocampus was measured by immunohistochemical method, and the activities or levels of SOD, GSH-Px, MDA in serum and brain tissue were determined. RESULTS: Comparing with model group, the mean escape latencies of rats were shortened and number of times that passed through original platform location increased in every dose SIF groups and positive control group (P<0.05), the mean escape latencies of rats in medium and high dose SIF groups were significantly shortened than that of positive control group (P<0.05). Result of immunohistochemistry revealed: comparing with model group, the integrated optical density of Aβ positive reaction product in every dose SIF groups and positive control group was decreased, and the Aβ-positive expression in high dose SIF group was significantly decreased than that of positive control group(P<0.05). Comparing with model group, the activities of SOD and GSH-Px in serum and brain tissue in medium and high dose SIF groups and positive control group were increased differently, and the content of MDA was decreased differently (P<0.05); The activity of SOD in serum and brain tissue in high dose SIF group was much higher than that of positive control group,and the content of MDA in high dose SIF group was much lower than that of positive control group (P<0.05); The activity of GSH-Px in serum in high dose SIF group was much higher than that of positive control group, but the activity of GSH-Px in brain tissue in high dose SIF group was no significant difference compared with positive control group (P>0.05). CONCLUSION: SIF could improve the behavioral capacity of AD model rats induced by Aβ_25-35, and the probable mechanism is that SIF changed the redox state, then increased the antioxidative level, thereby exhibited its neuro-protective effects.

Therapeutic effect of β-elemene on early optic neuritis in experimental allergic encephalomyelitis mice

ZHANG Rong-wei, CHEN Lei

2010, 15(10):  1128-1132. 

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AIM: To investigate the therapeutic effect of β-elemene on early optic neuritis in experimental allergic encephalomyelitis (EAE)mice. METHODS: The morphological changes of optic nerve in EAE group and β-elemene treated group were observed by morphology;the expression of β-amyloid precursor protein(β-APP)and 2,3-cyclic nucleopide 3-phosphdiesterase(CNPase) proteins were detected by immunohistochemistry. RESULTS: Compared with the EAE model group,the inflammation of optic nerve in β-elemene treated group was reduced, the expressions of CNPase were increased(31.8±1.0 vs 10.6±0.7, P<0.05)and the expressions of β-APP were decreased(80.4±9.4 vs 182.7±9.1, P<0.05)in β-elemene treated group. CONCLUSION: β-elemene can protect the optic nerve myelin and axon from the damage in EAE mice.

Effects of sirolimos on the expression of CD₈₆, I-A^b and TLR4 on mice dendritic cells

HE Xiu-juan, ZHANG Han, LI Dong-mei, QIU Yong, LONG Jun, AN Yun-qing, HU Yong-xiu

2010, 15(10):  1133-1138. 

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AIM: To observe the effects of sirolimos (SRL) on the maturation of murine bone marrow-derived dendritic cells (DC) and on the transcription of toll-like receptor 4 (TLR4). METHODS: (1) DCs derived from C57BL/6 murine bone marrow cells were induced by cytokines, and morphology development of DCs was observed by inverted microscope and scanning electron microscope. (2) The expression of CD_11c, CD₈₆ and MHC classⅡmolecule of DCs were assessed by flow cytometry after treatment with SRL, and the changes of these molecules after stimulation with lipopolysaccharide (LPS). (3) The TLR4 mRNA transcription was detected by real-time PCR after treating with or without SRL. RESULTS: (1) Typical morphology of DCs can be observed during the different stages. (2) SRL inhibited the expression of CD₈₆, I-A^b of DCs after stimulation with LPS. (3) The transcription of TLR4 mRNA was increased in SRL group. CONCLUSION: DCs treated with SRL can resist to promoting the maturation of DCs by LPS. The expression of TLR4 mRNA could be increased by SRL.

A new method for median effective dosage measurement--improved robbins-monro process

NIE Li, PAN Wei, SONG Jian-guo

2010, 15(10):  1139-1142. 

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AIM: A new method to measure median effective dosage based on the principle of sequential trial was designed: improved robbins-monro process. To explore the reliability, advantage and disadvantage of the method. METHODS: Median lethal doses(LD₅₀) of procaine and pentetrazole were detected and compared with improved robbins-monro process and Classical Bliss method. RESULTS: LD₅₀ of procaine and pentetrazole with the improved robbins-monro process were 207.5 mg/kg and 107.2 mg/kg respectively, LD₅₀ with the Bliss method were 206.1 mg/kg and 101.2 mg/kg respectively. Results indicated that the LD₅₀ with improved robbins-monro process is similar with Bliss method, while the new method is fewer animals and time consuming and simpler to calculate. CONCLUSION: Improved robbins-monro process is suitable to mesure median effective dose, for example LD₅₀ or ED₅₀, for the drug which efficicy is rapid and point is easy to determine the end.

Correlation study of PXR^*1B polymorphisms and steady-state trough concentration and its antihypertensive effect of amlodipine

ZHANG Gui-xiang, YUAN Hong, YANG Guo-ping, HUANG Zhi-jun

2010, 15(10):  1143-1147. 

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AIM: To investigate the effect of PXR11193T＞C,8055 C＞T and PXR^*1B (contain 11193T＞C and 8055C＞T) on the steady-state trough concentration and antihypertensive effect of amlodipine in essential hypertension patients. METHODS: Genotyping of PXR11193T＞C and 8055 C＞T were determined by pyrosequencing sequenator. PXR^*1B was analyzed by PHASE V.2.1. 62 essentialhypertension patients rolled the clinical trial. They were treated with amlodipine for 8 weeks, according to once a day, 5 mg per time in every morning. Blood pressure was measured at 0, 4, 8-week. At 8-week the venous blood samples were collected before amlodipine administration. Plasma was used to determined concentration of amlodipine by HPLC-MS/MS. Statistical analyses were performed by the SPSS 13.0 software for Windows. RESULTS: 61 of patients finished the clinical trial and the effective power of amlodipine was 63.9％. There was no significantly difference of the steady-state trough concentration and antihypertensive effect of amlodipine among genotypes of PXR11193T＞C,8055 C＞T and PXR^*1B(P＞0.05). CONCLUSION: PXR polymorphisms does not significantly affect the steady-state trough concentration and antihypertensive effect of amlodipine in essential hypertension patients.

Analysis on the influencing factors of cerebral infarction patients with aspirin prevention failure

MAO Xin-lei, HAN Li-ya, HUANG Xiang-dong, GUAN Chao-hong, YAN Xue-qin, CHEN Wei-dong

2010, 15(10):  1148-1151. 

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AIM: To investigate the influencing factors of cerebral infarction patients with aspirin prevention failure. METHODS: 139 cerebral infarction patients with aspirin prevention failure and 24 community health examination cases who were regularly administered aspirin for long-term, were enrolled in the study. Everybody's medical history was recorded. Medical examinations included the measurement of weight, height, fasting blood lipid, glucose concentrations, and blood routine. The concentration of urinary 11-dehydrothromboxane B₂ of 60 patients without atrial fibrillation and 24 community health examination cases were determined. The clinical influencing factors of patients with aspirin prevention failure were compared with the controls. RESULTS: As compared with the controls, there were statistically significant differences in percentage of male, smoking, previous cerebral infarction, atrial fibrillation, high-density lipoprotein cholesterol concentrations and urinary 11-dehydrothromboxane B₂ concentrations(P<0.05). The results of logistic regression showed that there were significant correlation between aspirin failures and previous cerebral infarction, urine 11-DH-TXB₂ concentrations. CONCLUSION: It is suggested that male, smoking, previous cerebral infarction, atrial fibrillation, low high-density lipoprotein cholesterol are risk factors of aspirin failures. Aspirin resistance is an important risk factor for aspirin failures.

Effects of rosuvastatin on serum soluble cell adhesion molecule levels in patients with chronic heart failure

HU Li-qun, ZHANG Qi, CHEN Li, LI Hong-qi, YI Chang-sen

2010, 15(10):  1152-1155. 

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AIM: To observe the effects of short-term treatment of rosuvastatin on soluble E-selectin (sE-selectin) and P -selectin (sP-selectin) in patients with congestive heart failure(CHF). METHODS: 70 patients with CHF were randomly divided into conventional treatment group and intervention group, the conventional treatment group received placebo and the intervention group received rosuvastain 10 mg /n for 4 weeks. The levels of sE-selectin and sP-selectin were measured before and after treatment by ELISA. RESULTS: The levels of sE-selectin and sP-selectin in serum of patients with heart function class Ⅳ were significantly higher than those in patients with heart function classⅡand Ⅲ(P<0.01),but there was no significant difference between classⅡand Ⅲ (P>0.05).The levels of sE-selectin and sP-selectin in two groups were significantly decreased after treatment (P<0.05 or P<0.01), compared with the conventional treatment group, only the sE-selectin level was significantly decreased (P<0.05). CONCLUSION: The levels of sE-selectin and sP-selectin are significantly increased along with heart function deterioration.The level of sE-selectin is decreased with short-term rosuvastatin treatment.

Analysis of epidemiology and drug resistance of bacterial infection during the early postoperative period in liver transplantation

TIAN Yu-wei, ZHOU Jie, TAN Yong-fa, KAN He-ping, TANG Bin

2010, 15(10):  1156-1160. 

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AIM: To investigate epidemiology and drug resistance associated with bacterial infection during early postoperative period after liver transplantation in the purpose of prevention and treatment. METHODS: Altogether 163 recipients of liver transplantation admitted from Apr.2004 to Dec.2009 in Nanfang Hospital were studied retrospectively and identified the bacterial and drug resistance. RESULTS: Bacterial infections occurred in 86 cases(52.7%)and 166 strains separated from various specimens.The main sites for pathogens were respiratory tract(68.2%), then blood(9.6%) and urine(8.4%). Main infections developed in the first month, especial in the front two weeks (81.9%). The strains bacteria included 76G^- strains (46.2%),68 G⁺ strains (39.3%) and 24 fungi strains(14.5%). The most commom were strains Acinetobacter Baumannii, Staphylococcus haemolysis, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus coli (53.2%). There were 46 patients suffered from mixed bacterial infection. Most of the G^- bacteria were sensitive to carbapenems; G⁺ bacteria were sensitive to aminoglycosides and oxazolinones. CONCLUSION: The infective complications are common in patients with live transplantation an the early stage, it is important to identify the bacteria and drug resistance for the purpose of prophylaxis and treatments.

Correlation between Apolipoprotein CⅠ gene polymorphisms and lipid-lowering efficacy of atorvastatin

FAN Lei, SONG Hong-tao, XU Rong-qing, XU Zheng-wei

2010, 15(10):  1161-1165. 

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AIM: To investigate the effect of polymorphisms in ApoCⅠ gene on the treatment efficacy of small dose of atorvastatin on hyperlipidemia. METHODS: 94 cases in 387 patients with hyperlipidemia were treated with oral administration of atorvastatin 10 mg/d. The TC,TG, LDL-C,apoA1 and apoB were measured before treatment and after 4,8,12 weeks of treatment. The polymorphisms of ApoCⅠgene on HpaⅠlocus were determined by using PCR- RFLP method. RESULTS: In 387 patients, the relative frequency of H2 allele and H1 allele were 0.3165 and 0.6835,respectively. After 8 weeks of treatment, the decreasing in level of LDL-C was more prominent in non H2 carriers than that in H2 carriers (P<0.05),but this difference was not found in TC, TG and ApoB. CONCLUSION: The efficacy of lipid-lowering therapy was no difference in Apo CⅠ gene polymorphisms.

Clinical character of patients relapsed after withdrawal with lamivudine therapy

PENG Guan-qing, ZHANG Chang, LIU Xue-feng

2010, 15(10):  1166-1169. 

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AIM: To investigate the clinical character of patients who had achieved complete or partial response relapsed after withdrawal with lamivudine therapy. METHODS: 160 patients with complete response and 35 patients with partial response continue to be monitored after withdrawal with lamivudine therapy.The liver function was checked once a month, the levels of HBV DNA, HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were tested once every 3 months. And detailed record of the condition and re-treatment outcomes. RESULTS: The relapse rates of HBV-DNA were 30.6% in the complete response group and 85.7% in the partial response group. The difference was statistically significant (χ²=36.164, P<0.001). ALT recurrence rate were 28.75% (46/160) and 82.9% (29/35), the difference was statistically significant (χ²=32.645, P<0.001). In 6 months after withdrawal, the relapse rates of the patients were 80% in the complete response group and 53.1% in the partial response group. With a long course, the recurrence rate was relatively low. The relapsed patients who had achieved complete response were mild. Lamivudine withdrawal with the long-term follow-up, timely treatment, prognosis was good. CONCLUSION: The relapse rate of the patients who had achieved complete response is lower. The state of illness is better in the patients who had achieved complete response, whereas, the treatment of the patients who had achieved partial response with lamivudine should not be discontinue.

Structure of hepatitis B virus core protein and disablement in the hepatitis B antiviral therapy

LIU Xue-ni, PAN Qing-chun, ZANG Guo-qing, YU Yong-sheng

2010, 15(10):  1170-1173. 

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Core protein which is encoded by the hepatitis B virus (HBV) c gene can package the pre-genome mRNA to form nucleocapsid and play a key role in the HBV replication. The disablement of core protein by RNA interference, core protein antibody and core protein mutant can block capsid formation, inhibit HBV replication. This can be used as a new target for hepatitis B antiviral therapy.

Advance in research for pharmacokinetics and drug interactions of licorice

CHEN Jiang-fei, XU Ping, ZHU Su-yan, HU Yi-jian

2010, 15(10):  1174-1182. 

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Licorice is one of the oldest and most frequently employed botanicals in Chinese medicine with multiple pharmacological effects, and a wide range of clinical application. But licorice can also cause adverse effects and drug interactions. A clear understanding of the chemical composition, disposition and drug interactions of licorice is contributed to promote its usage rationally and safely in clinic. In this paper, pharmacokinetics and drug interactions of licorice are reviewed and discussed in recent several decades, in order to get a more comprehensive understanding about licorice and ensure the safety of clinical medication.

Research advances of the effects of hydrogen sulfide on central nervous system

LI Kang-qi, ZHANG Guang-qin

2010, 15(10):  1183-1188. 

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In mammalian central nervous system (CNS), hydrogen sulfide (H₂S) is predominately formed from the amino acid cysteine by the action of cystathionine β-synthase (CBS) and the biosynthesis of H₂S is regulated by the intracellular concentration of calcium and S-adenosylmethionine which acts as an allosteric activator of CBS.A rapidly accumulating body of evidence is now available suggesting that H₂S acts as a gaseous mediator or gasotransmitter in mammalian CNS.Reported physiological functions of H₂S include maintenance of calcium homeostasis, potentiation of long-term potentiation (LTP), suppression of oxidative stress, and modulation of neurotransmission.H₂S, functions as a neuromodulator, exerts both potentiates or inhibits biological effects through a variety of mechanisms.Besides its pro-reducing properties, H₂S is able to stimulate the production of cAMP, active ATP-activated potassium channels,and increase intracellular calcium.H₂S is also involved in the pathology of central nervous system (CNS) such as stroke and Alzheimer's disease.Latest studies suggest controlling the content of H₂S can play a protective role in the treatment in some disease models. H₂S holds the potential of therapeutic value for CNS diseases.

CONSORT 2010 Statement: New version guidelines for reporting parallel group randomised trials

LIU Yu-xiu, CHENG Qi, LIU Li-xia

2010, 15(10):  1189-1194. 

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The CONSORT statement which published first in 1996 meant a consolidated standards of reporting trials and comprised a checklist and flow diagram. The checklist and diagram were primarily intended for use in writing, reviewing or evaluating reports of parallel group randomized trials. The statement was a continually-evolving document. The revised CONSORT statement was presented in 2001 and 2010. More than 400 journals and three leading editorial groups across the world have now given their official support to CONSORT. The latest version, CONSORT 2010, improves the specificity and clarity of the previous checklist. Based on introducing the background, processes of forming CONSORT statement and reviewing the contents and requirements of CONSORT, we prepared this paper, hoping to improving the quality of reporting randomized controlled clinical trials in our country. Some comments and suggestions were given at the same time.

Preclinical advances in the abnormal expression and its activity of isoenzyme of superoxide dismutase

WANG Zhan-qing, WANG Jin-ping, LIU Jing

2010, 15(10):  1195-1200. 

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The expression and activity of isoenzyme of superoxide dismutase (Cu, Zn-SOD, Mn-SOD and EC-SOD) are very complicated. The different pathogenesis or physiology could lead to the change of their expression and activity in the different tissues or organs. And the amount and activity of isoenzyme of superoxide dismutase changes correspondingly. The reports on the abnormal expression and activity of isoenzyme of superoxide dismutasethe were rarely seen in domestic researches. This paper reviewed the preclinical reports on the enzymes. It would provide evidences for the diagnosis and therapy of the disease, the research of new drugs and the research of anti-oxide and anti-apoptosis.