The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2010, Vol. 15 ›› Issue (11): 1245-1250.

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The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography

M'boungou Gwladys Jysmard, WANG Xin-ting, LIU Xiao-dong

Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2010-07-14 Revised:2010-08-12 Online:2010-11-26 Published:2020-09-16
Contact: LIU Xiao-dong, professor, dedicated to the research of pharmacokinetic lesearch of new drug, drug transport and blood brain barrier, pharmacokinetics of herbal medicine. Tel: 025-83271006 E-mail: xdliu@cpu.edu.cn
About author:M'boungou Gwladys Jysmard, graduate student from the Republic of Gongo, dedicated to the pharmacokinetics research. E-mail: mboungougwla@yahoo.fr
Supported by:
This work is supported by the National Science Foundation of People's Republic of China(Grants 30873123, 81072693) and the Fundamental Research Funds for the Central Universities(Grant JKY2009009)

Abstract

Abstract: AIM: To establish a convenient method to analyze 3-O-Methyl-D-Glucose (3-OMG) in rat plasma using 1-phenyl-3-methyl-5-pyrazolone (PMP) as a pre-column derivatizing agent, and in the mean time using this method to analyze the 3-OMG in rat plasma. METHODS: 3-OMG reacted easily with PMP in the presence of 0.4 mol/L NaOH at a 70 ℃ water bath. The derivatives were analyzed by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and monitored by ultraviolet detector at 245 nm. RESULTS: All linear coefficients were over 0.991, and the quantitative limit was 0.0078 mg/mL. The recoveries were found to be 98%-105%. Variations of intra- and inter-day precision were less than 12%. The method was successfully applied to the analysis of 3-OMG in rat plasma. CONCLUSION: This method is simple and effective, and can be adopted to analyze the 3-OMG in the plasma from both normal and diabetic rats.

Key words: 3-O-methyl-glucose, 1-phenyl-3-methyl-5-pyrazolone, Derivatization

CLC Number:

R969.1

M'boungou Gwladys Jysmard, WANG Xin-ting, LIU Xiao-dong. The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(11): 1245-1250.

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The assay of the 3-o-methyl-d-glucose (3-OMG) in plasma with derivatization method by High Performance Liquid Chromatography

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