Abstract: AIM: To develop a method for the determination of muscone in plasma of the rat administered by artificial-musk. METHODS: Sample was prepared by liquid-liquid extraction,which was chromatographed on ACQUITY UPLC/Q-TOFMS with a ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm,1.7 μm)column at 35 ℃. The mobile phase was 85% acetonitrile with 0.1% formic acid-15% 0.1%FA at the flow rate of 0.2 mL/min. APCI ion source was used and data was collected at positive ion mode. RESULTS: A good linear relationship was obtained between the peak area ratio of muscone(R=0.9980) and the concentration of muscone over the range of 0.0756 to 3.78 μg/mL. The limit of quantitation was 0.05 μg/mL. Accuracy and precision of all concentrations meet the biological sample testing requirements(RE<10%,RSD<15%). Average absolute recovery was bigger than 81%. CONCLUSION: The method was reliable, stable, special and suitable for the study of preclinical pharmacokinetics of artificial-musk.

Key words: Artificial-musk, Muscone, UPLC/Q-TOFMS, Pharmacokinetics