Table of Content

Volume 15 Issue 2
26 February 2010

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Effects of H₂O₂ and 11,12-EET in EDHF mediated relaxation in the rat basilar arteries

SONG Biao, CHEN Zhi-wu, FAN Yi-fei

2010, 15(2):  121-125. 

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AIM: To study the effects of Hydrogen Peroxide(H₂O₂)and 11,12-epoxyeicosatrienoic acid (11,12-EET)on EDHF-mediated relaxation in the rat basilar arteries. METHODS: The relaxant effects of acetylcholine (ACh), H₂O₂, 11,12-EET, and catalase (CAT) on rat arteria basilaris in vitro were detected by vasomotoricity experiment in vitro. RESULTS: In the rat basilar arteries, preconstricted by 30 mmol/L KCl in vitro, ACh(10^-7-10^-4.5 mol/L) had the concentration-dependent relaxation effect. 3×10^-5 mol/L Nω-nitro-L-arginine-methyl-ester (L-NAME) and 10^-5 mol/L indomethacin (Indo) could partly inhibit the relaxation effect of ACh to the rat basilar arteries, but non-No/non-PGI₂-mediated relaxation was still significant (P<0.01 vs Vehicle). After using L-NAME and Indo,11,12-EET didn't produce relaxation effect in rat basilar arteries and H₂O₂ has little relaxation effect. CAT could not inhibit the relaxation effects of ACh. CONCLUSION: Our data suggest that H₂O₂ and 11,12-EET may not involve EDHF-mediated relaxation to ACh in the rat basilar arteries.

Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration

YAN Mei-di, YE Meng, LIN Lei, NI Shu-min, WANG Shao-min, LIU Shu-rong

2010, 15(2):  126-129. 

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AIM: To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS: 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS: It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-α and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION: Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro.

Protective effects of cilostazol on the chronic ischemic brain injury in mice and its action mechanism

YE Yi-lu, ZHANG Qi, ZHANG Jian-ting, ZHANG Xiao-hua, YU Yue-ping, WEI Er-qing

2010, 15(2):  130-134. 

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AIM: To observe the protective effects of cilostazol on chronic ischemic brain injury in mice and the relationship to angiogenesis. METHODS: Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). Cilostazol (10 mg/kg) was i.p. injected at 1, 4, 7 h and 1-14 d once a day after ischemia. The neurological deficits, angle in inclined board test, infarct volume, neuron density, and the expressions of VEGF and Flk-1 in ischemic region were determined at the 35 d after ischemia. RESULTS: Cilostazol (10 mg/kg) significantly reduced the neurological deficits and infarct volume, and increased the angle in inclined board test, the neuron density, and the expressions of VEGF and Flk-1. CONCLUSION: Cilostazol has neuroprotective effect on chronic ischemic brain injury in mice, which may relate to angiogenesis via inducing the expressions of VEGF and Flk-1.

Experimental study on preventive effects of benthiactzine against respiratory failure induced by cholinesterase inhibitor in rats

MO Chen, LONG Chao-liang, WANG Ru-huan, WANG Hai

2010, 15(2):  135-139. 

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AIM: To investigate the preventive effects of benthiactzine against respiratory failure induced by cholinesterase inhibitor DDVP in rats. METHODS: 40 healthy Wistar male rats were divided into 4 groups randomly: poisoning group, benthiactzine 0.5, 1.0 and 2.0 mg/kg preventive groups. Rats were poisoned with DDVP (i.p.) for 30 min after benthiactzine was injected beforehand. The symptoms, respiratory rate, blood gas analysis and the pathological change were observed. RESULTS: After DDVP poisoning, the rats of poisoning group all died within 3-5 min with the symptoms of fasciculation, cyanosis and convulsion et al. The rats of benthiactzine preventive groups showed mild N symptoms such as fasciculation and weakness, and then recovered. The blood gas analysis demonstrated no damages caused by the poison. The lung tissue slides of poisoning group with HE staining showed that the pulmonary vessels were dilated and filled with erythrocytes, pulmonary intervals became wide and red blood cells could be seen in alveolar and bronchial cavities, and many inflammatory cells accumulated, pulmonary edema and hemorrhage appeared. The lung tissues of rats in preventive groups were normal. CONCLUSION: Benthiactzine can effectively prevent respiratory failure induced by DDVP.

Pioglitazone decrease the level of oxidative stress of insulin resistance

LI Lan-fang, GUO Yu, TANG Guo-tao, CHEN Shu-xian, CHEN Lin-xi

2010, 15(2):  140-144. 

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AIM: To investigate the effects of pioglitazone on oxidative stress and insulin resistance. METHODS: The insulin resistance cell model was induced by TNF-α(4 ng/mL)and high dose of insulin(10^-7 mol/L) to stimulate human hepatoma carcinoma cell HepG2. The cytotoxicity of HepG2 was observed by MTT. The concentration of glucose in cell culture fluid of HepG2 was detected using a glucose oxidase assay kit. The level of intracellular reactive oxygen species (ROS) was detected by DCFH-DA fluorescent probe and flow cytometry. The translocation of NF-κB was observed by immunofluorescence. RESULTS: Compared with control, TNF-αand high dose of insulin treatment significantly increased the level of ROS in HepG2 and the concentration of glucose in the cultrure medium of HepG2. However, pioglitazone treatment could reverse those effects of TNF-α and high dose of insulin. NF-κB translocated to nuclear stimulated by TNF-αand high dose of insulin. Pioglitazone could inhibit the translocation of NF-κB. CONCLUSION: Pioglitazone improve the insulin resistance condition by degrading oxidative stress level and inhibiting the translocation of NF-κB.

CpG ODN attenuates airway inflammation and modulates the expression of signal transducers and activators of transcription 6 in asthmatic mice

YANG Yuan, PENG Xiao-hua, LIN Yong

2010, 15(2):  149-153. 

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AIM: To investigate the effects of CpG ODN on the airway inflammation and the expression of signal transducers and activators of transcription 6(STAT6) in asthma. METHODS: Thirty-two male BALB/c mice were randomly divided into 4 equal groups: control group,asthma group, DXM group and CpG ODN group ,the mice model of asthma was established by the ovalbumin(OVA) challenge methods. The lung tissue was gained from the left lung, bronchoalveolar lavage fluid(BALF) was obtained from the right lung. The total cell numbers,eosinophils(EOS) numbers in BALF were counted by different count fluids; Left lung tissue was stained by HE and the changes of inflammation were observed. The protein expressions of STAT6 were detected by immunohistochemistry. RESULTS: The total cell numbers in BALF, the absolute numbers of EOS were all significantly higher in asthma group than those in the control group(P<0.05); the total cell numbers in BALF, the absolute numbers of EOS were all significantly lower in DXM group and CpG ODN group than those in asthma group(P<0.05), CpG ODN and DXM attenuated the airway inflammation of asthmatic mice. The expressions of STAT6 were mainly on the bronchial epithelium. STAT6 protein levels were increased in asthma group. CpG ODN significantly reduced STAT6 protein expression. DXM significantly reduced the levels of STAT6 protein. There was a significant positive correlation between the absolute numbers of EOS in BALF, the content of STAT6 in the epithelial cells(r=0.748). CONCLUSION: CpG ODN and DXM can extenuate the progression of airway inflammation following down regulated allergen challenge via regulation of STAT6/IL-4 signal pathway.

Effects of total glucosides of paeong on improving insulin sensitivity, lowering blood pressure and enhancing antioxidation in metabolic syndrome-hypertensive rats

FENG Rui-er, ZHENG Lin-ying, LV Jun-hua, PAN Jing-qiang, XIAO Liu-ying

2010, 15(2):  154-159. 

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AIM: To investigate the antihypertensive effects and effective mechanisms of total glucosides of paeong(TGP for short)on insulin resistance, hypertension,and the changes of oxidative stress in metabolic syndromic(MS)-hypertensive(Ht)rats. METHODS: The SD rats were fed with a high fat(25%)-sucrose (10%)-enriched diet qd for 8 weeks. When the rats were exposed hypertension and impaired glucose tolerance (IGT),and the rats were randomly divided into normal control group, metformin (Met) group 200 mg/kg, and TGP 150, 50 mg/kg, solvent or the drugs were adiministered by intragastric administration (i.g.) qd for 4 weeks. The blood pressure (BP), the contents of fasting blood glucose (FBG), 2-hours blood glucose after oral glucose tolerance test (2 h BG-OGTT), and fasting insulin (Fins) were determined, as well as the insulin sensitivity index (ISI) and insulin resistance index (HOMA-IRI) were calculated; the contents of free fatty acids (FFA), TNF-α, endothelin (ET-1), renin-angiotensinⅡ, NO,malonaldehyde (MDA) and activities of nitric oxide synthase (NOS) and superoxide dismutase (SOD) activity were determined after treatment. RESULTS: Compared with normal control group,the BP and the contents of FBG,OGTT-2 h BG,Fins were incresaed in MS-Ht rats,and ISI was weaken, HOMA-IRI was incresaed. the contents of FFA,TNF-α,ET-1,renin-Ang Ⅱ,MDA were incresaed;the content of NO,the activities of NOS and SOD were decreased(P<0.05 or P<0.01). The ISI was incresaed,the HOMA-IRI was decreased,retrieve hyperinsulinemia(P<0.05 or P<0.01), the contents of FFA,TNF-α,ET-1,renin-Ang Ⅱ,MDA were decreased,the activities of NO,NOS,SOD were elevated in Met group and TGP 150 mg/kg group. Compared with TGP 150 mg/kg group,the contents of FBG,OGTT-2 h BG were decreased in Met group,there was no significant difference. CONCLUSION: TGP and Met are able to enhance the insulin sensitivity, retrieve hyperinsulinemia,Antagonism of ET-1, renin-Ang Ⅱ system and oxidative stress response,augment the function of NO, NOS, degrade the BP;TGP does not have a strong predominance against hyperglycaemia, TGP differs from Met. The effects of TGP show dose-effect relationship.

Protective effects of DDPH on myocardial ischemia reperfusion injury in isolated rat hearts

ZHANG Hong-xia, WU Yong-jie, GAO Ming-tang

2010, 15(2):  160-165. 

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AIM: To investigate the protective effect of 1-(2,6-dimethylphenoxy)-2-(3, 4-dimethoxyphenylethylamino) propane hydrochloride (DDPH) on myocardial ischemia-reperfusion (I/R) injury in isolated rat hearts. METHODS: Myocardial ischemia-reperfusion injury models were built with Langendorff isolated perfusion technology and established by the ligation of left descending coronary artery (LAD) for 40 min and reperfusion for 120 min in isolated rat hearts.The influence of DDPH on left ventricular function, myocardial infarct size, lipid peroxidation and the ultramicrostructural changes of myocardial cells were observed. RESULTS: DDPH improved the damage of left ventricular systolic and diastolic function caused by I/R, obviously reduced myocardial infarct size, increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), decreased the content of MDA in myocardium, and lessen the degree of ultramicrostructural injury in myocardial cells. CONCLUSION: DDPH has a protective effect on myocardial ischemia-reperfusion injury in isolated rat heart, which may be related to inhibiting the formation of the oxygen free radical and subsequent lipid peroxidation.

Dynamic change of ERKmRNA and phosphorylation protein level in rats with asthma

ZHANG Jin-bo, WU Cheng-yun, LI Zhi-qiang, DAI Yuan-rong

2010, 15(2):  166-169. 

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AIM: To explore the dynamic changes of ERKmRNA and phosphorylation protein level in rats with asthma. METHODS: The rats were divided into control group,asthma (0.5,1,3,12 h)groups and the peripheral blood mononuclear cells(PBMCs)of rats were cultured and collected. The expressions of ERKmRNA and phosphor protein of ERK in PBMCs of all groups were detected by RT-PCR,Western bloting, respectively. RESULTS: Compared with control group,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma (0.5,1,3 )groups (all P<0.01). There was no difference between asthma(12 h)and control group. Compared with control group,asthma (0.5,3,12 h)groups,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma(1 h)group. CONCLUSION: Phosphorylation ERK may play an important role in airway inflammation and remodeling of asthma,deeply studying the ERK's specific regulation results in the pathogenesis of asthma may have an important significance on the prevention and treatment clinical asthma.

Research of the mechanism about proanthocyanidins’s diastolic function to rat resistance vessel

WANG Yan-feng, PENG Xiao-hui, ZHANG Chen-yao, ZHAO Xin, WANG Yan-ying

2010, 15(2):  170-174. 

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AIM: The present study was designed to investigate the relaxing effects and mechanism of PRF in rat mesenteric arterial bed(MAB) and isolated mesenteric artery(MA). METHODS: Isolated the MAB and MA of the rat,and the MAB and MA were cuted into artery rings, the tension of MAB and MA rings was measured by Myograph System. RESULTS: PRF could induce a concentration-dependent relaxation of MAB rings which was pre-contracted with phenylephrine(Phe), the relaxation rate was 70%(compared to the control). This effect was significantly reduced by the nitric oxide(NO) Synthase inhibitor N-nitro-L-arginine(L-NOARG) or high K⁺ solution and completely inhibited by KCl plus L-NOARG. But the vasorelaxant effect could not be altered by indometacin,atropine,yohimbine,pyrilamine K channel inhibitor: BACl₂, glibenclamide,ouabain,4-aminopyridine. In Phe pre-contracted MA rings, PRF also induced a concentration-dependent relaxation,which was inhibited by endothelial removal, charybdotoxin(ChTx), and ChTx plus apamin, respectively.Moreover the relaxant functiont was not altered by HOE140 and apamin given alone. CONCLUSION: The vasorelaxing effect of PRF is dependent upon the NO-cGMP pathway and in combinaton with hyperpolarization due to activation of Ca²⁺-dependent K⁺ channels.

UPLC/Q-TOFMS determination of muscone in plasma of rat administrated by artificial-musk

LENG Yu-jing, LI Hai-tao, DENG Hai-shan

2010, 15(2):  175-179. 

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AIM: To develop a method for the determination of muscone in plasma of the rat administered by artificial-musk. METHODS: Sample was prepared by liquid-liquid extraction,which was chromatographed on ACQUITY UPLC/Q-TOFMS with a ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm,1.7 μm)column at 35 ℃. The mobile phase was 85% acetonitrile with 0.1% formic acid-15% 0.1%FA at the flow rate of 0.2 mL/min. APCI ion source was used and data was collected at positive ion mode. RESULTS: A good linear relationship was obtained between the peak area ratio of muscone(R=0.9980) and the concentration of muscone over the range of 0.0756 to 3.78 μg/mL. The limit of quantitation was 0.05 μg/mL. Accuracy and precision of all concentrations meet the biological sample testing requirements(RE<10%,RSD<15%). Average absolute recovery was bigger than 81%. CONCLUSION: The method was reliable, stable, special and suitable for the study of preclinical pharmacokinetics of artificial-musk.

Comparison of the efficacy between rabeprazole sodium and omeprazole sodium for injection on 24-hour intragastric pH in healthy volunteers

WANG Jin, CHAO Yang, WANG Yong-qing, YU Feng

2010, 15(2):  180-184. 

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AIM: To compare the effects between different doses of rabeprazole(RAB) sodium for injection and 40 mg of omeprazole(OME) sodium for injection on 24-hour intragastric pH in healthy subjects. METHODS: In this open, paralleled, multi-center study, 84 healthy volunteers were divided into 4 groups. They were administered with intravenous dripping of 10 mg(Group A),20 mg(Group B) and 40 mg(Group C) per day of RAB and 40 mg(Group D) per day of OME respectively for 5 days. The values of intragastric pH of each subject were monitored for 24 h on the first day and the last day. RESULTS: The median 24 h intragastric pH values: the pH values on day 5 were significantly increased compared with those on day 1 (P<0.05). The mean percentage of the gastric pH>4 and pH>6 on day 5 were significantly increased compared with those on day 1 (P<0.05). The mean percentage of the gastric pH>4 in Group C were higher than those in Group A on day 1(P<0.05); and the mean percentage of the gastric pH>6 in Group D was higher than those in Groups A and B on day 5(P<0.05). CONCLUSION: Both RAB and OME sodium for injection have great inhibitory effect on gastric acid secretion. Continuous administration can more significantly inhibit the gastric acid than that of single-dose. The efficacy and drug potency of RAB 20 mg in gastric acid inhibition are similar to those of RAB 40 mg. The acid suppression is similar with single dose of RAB 10 mg, 20 mg, 40 mg and OME 40 mg. The roles of acid suppression are similar with continuous administration of three different doses of RAB. Besides, gender does not affect the pharmacodynamic action of the drugs.

Determination of lamivudine in human plasma by RP-HPLC and its application to bioequivalence research

SUN Hua, ZHAO Ya-nan, GUO Yi-yun, LI Xiang-hong, YANG Jing-jing, MAO Guo-guang, XIE Hai-tang

2010, 15(2):  185-189. 

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AIM: To develop a HPLC method for the determination of lamivudine in plasma and study the pharmacokinetics and relative bioavailability of domestic lamivudine tablet in Chinese healthy volunteers. METHODS: A single oral dose (0.3 g) of test tablet (domestic) or reference tablet (imported) was given to each volunteer according to an open randomized crossover study. The concentrations in plasma were determined by RP-HPLC method. The prepared sample was separated on the column of Kromasil C₁₈(250 mm×4.6 mmID,5 μm),with the mobile phase consisting of methanol-25 mmol/L potassium dihydrogen phosphate(containing of 1% triethylamine pH 3.3) (10∶90) at a flow rate of 1 mL/min. The eluted peaks were detected by UV detector at 280 nm. The pharmacokinetic parameters and relative bioavailability were calculated by DAS 2.1. RESULTS: The calibration curve was linear in the range of 25-7500 μg/L(r=0.9992). The relative recoveries of low, medium and high concentration were within 100.09%-103.36%. The intra-batch and inter-batch RSD were less than 2.2% and 7.8%, respectively. The main pharmacokinetic parameters of lamivudine were as follows: C_max were (2835±734) and (2868±726) μg/L;t_max were (1.15±0.60) and (1.05±0.78) h;t_1/2 were (2.68±0.32 ) and (2.58±0.41) h;AUC_{0-14 h} were (10994±1839) and (10593±1654) μg·L^-1·h;AUC_0-∞ were (11330±1908) and (10884±1734) μg·L^-1·h for test and reference preparations, respectively. The relative bioavailability of test tablet was(104.80±15.56)%. CONCLUSION: The assay method is simple, sensitive, accurate and good enough to be used in pharmacokinetic study of lamivudine. The results of statistical analysis show that two preparations in research are bioequivalent.

Effects of ATP-sensitive potassium channel opner iptakalim on deacclimatization to high altitude

CUI Wen-yu, NIE Hong-jing, ZHANG Dong-xiang, XIAO Zhong-hai, ZHANG Yan-fang, CUI Jian-hua, SHI Yong-ping, LONG Chao-liang, WANG Yin-hu, WANG Hai

2010, 15(2):  190-193. 

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AIM: To study the effects of iptakalim, a novel ATP-sensitive potassium channel opner, against deacclimatization to high altitude. METHODS: This study was performed with a randomized, double-blind, placebo-controlled, parallel-group design. Fifty-six male soldiers were randomly divided into two groups: treatment group (39 men) and control group (17 men). The subjects were taken iptakalim tablets (5 mg/tablet, once a day) or placebo(once a day) respectively,for 7 months. The treatment was stopped at the 2 days before the subjects return to 3400 m altitude, and then returned to plain (1400 m). The questionnaire of symptoms of deacclimatization to high altitude was used on the 2nd, 4th, 6th day after the subjects returned to 1400 m altitude and the scores of deacclimatization symptoms were evaluated. RESULTS: Among the 17 men staying at 5200-5380 m high altitude for a long time, the incidences of deacclimatization to high altitude were 82.4%, 52.9% and 23.5% on the 2nd, 4th, 6th day after they returned to 1400 m altitude,respectively. After treated with iptakalim for 7 months, the incidences of deacclimatization were 43.6%, 30.8% and 38.5%,respectively. The scores of deacclimatization symptoms of placebo-control group on the 2nd and 4th,6th day after returning to 1400 m were 6.0±1.9, 4.6±1.0 and 3.8±1.5 respectively, while those in iptakalim-treated group were 4.4±2.4, 3.3±1.8 and 4.0±1.5 respectively. Compared with control, symptoms of deacclimatization to high altitude of treatment group were reduced significantly on the 2nd and 4th day after returning to 1400 m. There was no statistically significant difference in scores of deacclimatization symptoms between the two groups on the 6th day. CONCLUSION: Iptakalim can decrease the incidence of deacclimatization to high altitude, mitigate the symptoms of deacclimatization to high altitude. Iptakalim have a significant effect on prevention and treatment of deacclimatization to high altitude.

Bioequivalence of domperidone tablets in healthy male volunteers

LI Ling, GAO Na, GU Juan, YAN Jin, YANG Guo-ping, OUYANG Dong-sheng

2010, 15(2):  194-198. 

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AIM: To evaluate the pharmacokinetics and bioequivalence of domperidone tablets in healthy volunteers. METHODS: A single dose (20 mg test or reference formation) was given to 18 healthy male volunteers according to an open randomized crossover design. The concentration of domperidone in plasma was determined by LC-MS. The pharmacokinetics parameters were calculated and the bioavailability and bioequivalence of two formulations were evaluated by DAS Ver 2.0. RESULTS: The main pharmacokinetic parameters of domperidone tablets were as follows: C_max were (36±8) and (38±9) μg/L; t_max were (0.6±0.2) and (0.7±0.3) h; t_1/2 were (10±8) and (9±3) h; AUC_0-24 were (138±34) and (144±31) μg·L^-1·h; AUC_0-∞ were (168±68) and (166±37) μg·L^-1·h. As assessed in accordance with AUC_0-24, the relative bioavailability of two formulations was (97±20)%. CONCLUSION: The domperidone formulations are bioequivalent.

Influence of atorvastatin on the curative effect and restenosis in patients with acute myocardial infarction for postoperative percutaneous coronary intervention

DENG Shao-xiong, ZHENG Yuan, GUO Nan-ou

2010, 15(2):  199-203. 

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AIM: To investigate the influence of different dose atorvastatin on the curative effect and restenosis in patients with acute myocardial infarction for postoperative percutaneous coronary intervention (PCI). METHODS: 162 patients with acute myocardial infarction for postoperative PCI were divided into Group A(atorvastatin 20 mg/d group) and Group B(atorvastatin 40 mg/d group), both of which were treated with atorvastatin for 12 months. Serum lipid, CRP, IL-6, NO, CGRP, ET-1, D%, liver function,renal function and CK were measured before PCI, 6 months and 12 months after PCI.In addition,the curative effects of atorvastatin,adverse cardiovascular outcomes and side effect of atorvastatin were observed. RESULTS: Compared with the Group A, the TC, LDL-C, CRP, IL-6, ET-1 were significantly reduced and the HDL-C, NO, CGRP, D% were improved in Group B after treatment(P<0.05), and there was no statistical difference(P＜0.05) with no severe side effect. In addition,the morbidity of restenosis after PCI in Group B was decreased, and there was no statistical difference(P＜0.05). CONCLUSION: High dose atorvastatin have a significantly anti-inflammatory effect and endothelial function improvement, decreasing significantly the morbidity of restenosis after PCI.

Controlled study on quetiapine and Risperidone in the treatment of elder schizophrenic patients

HU Heng-zhang, ZHANG Lian-zhong, WEN Jang-gang, HAO Jing-yu, WANG Li-yong

2010, 15(2):  203-205. 

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AIM: To research the therapeutic and side effects of Seroquel in the treatment of elder schizophrenic patients. METHODS: Thirty schizophrenic patients were treated with Risperidone as the controlled group.The efficacy was measured with the positive and negative symptoms scale (PANSS), while the adverse events were determined by the treatment emergent symptoms scale (TESS). RESULTS: The efficacy rate of Risperidone was 78%,in which 39% were improved markedly. The efficacy rate of Seroquel was 88%, in which 76% were improved markedly. CONCLUSION: It is suggested that both drugs are effective exactly in the treatment of schizophrenic patients and fewer side effects.Seroquel appears to be more effective in improving psychiatric symptoms.

Effects of simvastatin combining clopidogrel on high-sensitivity C-reactive protein and blood lipids in patients with acute coronary syndrome

QIAN Jian, XU Jian

2010, 15(2):  206-209. 

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AIM: To explore the effects of simvastatin and clopidogrel on high sensitivity C-reactive protein and blood lipids in patients with acute coronary syndrome. METHODS: 67 cases with acute coronary syndrome were randomly divided into intervention group (35 cases) and control group (32 cases).Two groups received the same conventional treatment, in addition, the intervention group was treated with simvastatin and clopidogrel, the levels of high sensitivity C-reactive protein and blood lipids were detected before and six week after treatment. RESULTS: The high sensitivity C-reactive protein and blood lipids levels of patients were significantly decreased in intervention group, compared with control group (P＜0.05). CONCLUSION: Treatment with simvastatin and clopidogrel not only decreases the blood lipids levels but also has the best effects on lowering high sensitivity C-reactive protein in patients with acute coronary syndrome.

Effect of mild hypothermia therapy on the concentration of plasma C-Reactive protein following acute severe brain injuries

CHEN Zai-feng , XU Xin-long, FU Xiao-jun, WEI Xiao-jie, PAN Hong-song, XIE Qin-song, SHI Bin-qun

2010, 15(2):  210-213. 

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AIM: To observe the effect of mild hypothermia therapy on concentration of plasma C-reactive protein following acute severe brain injuries and to explore the treatment effect of mild hypothermia on acute severe brain injury patients. METHODS: A total of 120 patients with acute severe brain injuries were divided into mild hypothermia therapy group and general group randomly. The patients of mild hypothermia therapy group were treated with mild hypothermia therapy besides general treatment. Serial concentrations of C-reactive protein in serum were measured in all patients from 6 h to 6 d after hospitalization. RESULTS: The concentration of C-reactive protein in serum of both groups were significantly higher than those of normal group(P<0.05); The concentration of C-reactive protein in serum of mild hypothermia therapy group were significantly lower than those of general group(P<0.05); The concentrations of C-reactive protein in serum in the group with poor prognosis were higher than those in the group with sound prognosis. CONCLUSION: The concentration of C-reactive protein in serum was elevated significantly after acute severe brain injuries, mildhypothermia could decrease the concentration of C-reactive protein in serum, protect the neurofunction and improve the prognosis.

Research progress of miRNA in diabetes

GONG Zhi-cheng, HUANG Qiong, LIU Zhao-qian

2010, 15(2):  214-218. 

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The discovery hundreds of small RNA molecules, called microRNA, with the potential to modulate the expression of the majority of the protein-coding genes has revolutionized in the diabetes field. Moreover, alterations in the level or function of microRNA are associated with an increasing number of diseases. We present evidence for the involvement of microRNA in diabetes mellitus, by outlining the contribution of these microRNA molecules in the control of pancreatic β-cell functions and by reviewing recent studies reporting changes in microRNA expression in tissues. MicroRNA holds great potential as therapeutic targets. In this article, the researches of miRNA in diabetes and their potentialfor molecular therapy were summarized.

Recent research progress of eIF3 in biological function

XU Xiao-jing, ZHOU Hong-hao, LIU Zhao-qian

2010, 15(2):  219-224. 

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eIF3a is the largest subunit of mammalian eukaryotic initiation factor 3 (eIF3). It has been shown by many research that eIF3a plays an essential role in regulating the initiation of mRNA translation and protein translation in mammalian cells as well as yeast cells.Additionally, eIF3a involves in cell cycle regulation, which also influents cell proliferation and differentiation greatly. There is a close relationship between eIF3a and tumor progression, development, prognosis and chemotherapeutic efficacy, moreover the genetic polymorphisms of eIF3a associates with susceptibility of carcinoma. This review summarizes the research progress of eIF3a biological functions in recent years.

Saponins in tumor therapy

SHANG Li-li, SUN Jian-guo, XIE Hai-tang, WANG Guang-ji

2010, 15(2):  225-232. 

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Saponins are structurally diverse compounds, most of which have amazing anti-tumorigenic effect. This review summarizes the role of saponins in tumor therapy, the mechanism of actions and their structure-activity relationship, thus giving some information to the futher mechanism research and new naturally occurring anti-tumor drugs discovery.

Recent advances of diosgenin in its pharmacologic activities and mechanism

YUE Lei, CHEN Ling, KOU Jun-ping, YU Bo-yang

2010, 15(2):  233-237. 

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Diosgenin, an important steroid sapogenin, is one of major precursors of synthetic steroids. It is rich in natural resources and has various significant activities such as anti-tumor, anti-inflammation, and cardiovascular-protection and so on. Recently, it causes increasing attention of scholars all over the world due to its valuable application in medicine. In this paper, thelatest international research advances of diosgenin in its pharmacologic activities and mechanism were reviewed.

Mediation of P-selectin in myocardial ischemia-reperfusion injury

JIN Kang, KAN Hong-wei

2010, 15(2):  238-240. 

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The process of myocardial ischemia-reperfusion injury (MI/RI) is complex and dynamic. Platelets,neutrophils and vascular endothelial cells are three key players in MI/RI, and P-selectin plays an important role in the transductive regulation among these players. This review summarizes the mediationof P-selectin among several kinds of materiaes in the process of MI/RI.