Inhibitions of articular cartilage cells apoptosis in rats with adjuvant arthritis by Amiloride, a blocker of acid-sensing ion channels

Abstract

Abstract: AIM: To investig ate the protective effects of articular cartilage by acid-sensing ion channels (ASICs) blocker A miloride and its mechanisms.METHODS: The rats were randomly divided into normal group, AIA model group, Amiloride (2.5, 5.0, 10.0 mg·kg -1·d-1) group, aspirin (50 mg/kg) group.The AIA model rats were induced by CFA.The secondary inflammat ion of AIA rats was appeared on the tenth day via intradermal injection CFA.The drug groups were given corresponding experimental drugs, the model group and the normal group were administered with the corresponding capacity of sterile water, lasting for eight day s.The right hind paw articular swelling degree was determined with volume meter, the morphological changes of articular cartilage was observed by light microscope.The apoptosis of articular cartilage cells were observed by transmission electron microscope, Tunel method. The typeII collagen (COII) expressions of extracellular matrix of articular cartilage were determined by using immunohistochemistry.The proteoglycan (PG) contents of the cartilaget issue were detected by Alcian blue staining.RESULTS: Amiloride have no conspicuous effect on the hind paw swelling with AIA rats.The pathology ical study showed that the surface of articular cartilage was slick, the layer was lampros and the quantity of chondrocyte was incresaed.Transmission electron micro scope study showed that condensation and crack of nucleus and apoptotic bodies were appeared in articular cartilage cells in model group, and the condensation of heterochromatin, the distention of rough endoplasmic reticulum and the swell of bioblast were relieved in Amiloride groups.The apoptosis cell calculation in the experiment groups were fewerthan those in the model group (P <0.01) by Tunel method.The COII and PG of extracellular matrix of articular cartilage were significantly increased in Amiloride groups by immunohistochemical method and Alcian blue staining.CONCLUSION: ASICs blocker Ami loride may have protective effects on articular cartilage by inhibiting the apoptosis of articular cartilage cells of adjuvant-induced arthritis rats.

Key words: Amiloride, Adjuvant-induced arthritis(AIA), Acid sensing ion channels(ASICs), Apo ptosis

CLC Number:

R965.2

ZHANG Teng-yue, CHEN Fei-hu, WANG Nian, YUAN Feng-lai, HU Wei, WU Fanrong. Inhibitions of articular cartilage cells apoptosis in rats with adjuvant arthritis by Amiloride, a blocker of acid-sensing ion channels[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(1): 41-46.

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