Endoxin mediating myocardial ischemia reperfusion injury by effecting expression of apoptosis and inflammation genes

Abstract

Abstract: AIM:To observe the changes of cardiac muscle apoptosis and inf lammation related gene expression in rats with myocardial ischemia reperfusion (MIR)injury utilizing signal transduction gene array, and the effects on the rats after injecting antidigoxin, endoxin specificness antagon.It was testified that endoxin can mediate MIR injury by influencing apoptosis and inflammation related gene, and the mechanism of action of endoxin mediating MIR injury was consummated.METHODS:Myocardial ischemia reperfusion models were obtained by ligating left anterior descending coronary artery 30 minutes, followed by 60 minutes reperfusion.SD rats were randomly divided into three groups each with three rats :sham operation group,MIR group and antidigoxin antiserum group.After reperfusion, left ventricular myocardium samples of ischemia area were immediately processed, and the expression of related apoptosis and inflammation genes were measured with gene array technology.RESULTS:Compared with sham operation group, the apoptosis related gene expression of Bax, Bcl-2, Bcl-2L1 and Birc1b in MIR group were down regulated, but the ratio of Bcl-2/Bax was descended.The mediated inflammation related gene expression of IL, TNF, ICAM-1 were up-regulated or had the tendency of up-regulation.Compared with MIR group, the apoptosis related gene expression of Bax, Bcl-2, Birc3 in antidigoxin antiserum group were up-regulation, but the ratio of Bcl-2/Bax was upgraded.The mediated inflammation related gene expression of IL, TNF, ICAM-1 were down regulated or had the tendency of down regulation.CONCLUSION:Endoxin can down regulate the inhibition apoptosis gene expression and up-regulate inflammation gene expression.Antagonist of endoxin, antidigoxin antiserum can rivalry endoxin.It has myocardial preservation protection by down regulating the related gene of myocardium apoptosis expression and up-regulating inflammation related gene expression.

Key words: endoxin, ischemia reperfusion injury, myocardium, gene, apoptosis, inflammatory factor

CLC Number:

R965.2

ZHENG Jian-fa, KE Yong-sheng, GAO Wen-jun, WANG He-gui. Endoxin mediating myocardial ischemia reperfusion injury by effecting expression of apoptosis and inflammation genes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 880-885.

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