Effects of noninvasive delayed limb ischemic preconditioning on oxidative injury following myocardial ischemia /reperfusion in diabetic rats

Abstract

Abstract: AIM: To study the protection of noninvasive delayed limb ischemic preconditioning (NDLIP) on myocardial ischemia/reperfusion oxidative injury in diabetic rats. METHODS: Diabetic rat models were induced by injecting streptozotocin (STZ) into the vena caudalis. The diabetic rats were divided randomly into three groups: myocardial ischemia/reperfusion (I/R) group, myocardial ischemic preconditioning (MIP) group and noninvasive delayed limb ischemia preconditioning (NDLIP) group. The rats were pretreated with three cycles of 5 min ischemia /5 min reperfusion on the left hind limb, once a day for three consecutive days, to establish the NDLIP models. And MIP models were subjected to three episodes of 5 min of ischemia coupled to 5 min of reperfusion in the left anterior descending (LAD) coronary artery of rats. The models of I/R were established by 30 min ischemia and 120 min reperfusion of LAD in rats. The electrocardiogram was monitored continuously to record ventricular arrhythmia (VA) during 30 min ischemia. I/R-induced infarct size (IS) was determined using triphenyltetrazolium chloride (TTC) staining. Myocardial total superoxide dismutase (T-SOD) and manganese superoxide dismutase (Mn-SOD) activity, and malondialdehyde (MDA) content were determined respectively in each group rats. RESULTS: Compared with group I/R, the onset of ventricular premature contraction (VPC) was delayed markedly (P<0.01), duration of VPC was shortened (P<0.01), incidences of ventricular tachycardia (VT) and ventricular fibrillation (VF) were decreased (P<0.01), myocardial IS was diminished (P<0.01), infarct size /area at risk(IS AAR) was degraded, myocardial MDA content was declined (P<0.01), myocardial T-SOD and Mn-SOD activity were increased (P<0.01) in group MIP, group NDLIP.CONCLUSION: Noninvasive delayed limb ischemic preconditioning can improve the myocardial antioxidation of diabetic rats.

Key words: limb, remote ischemic preconditioning, diabetes mellitus, ischemia /reperfusion injury, antioxidation, heart protection

CLC Number:

R965.2

LI Yu-mei, ZHU Xue-hui, YUAN Heng-jie, LUO Qiong, WU Yan-na, KAN Yi, JIAO Jianjie, GAO Wei-zhen, LIU Yan-xia, LOU Jian-shi. Effects of noninvasive delayed limb ischemic preconditioning on oxidative injury following myocardial ischemia /reperfusion in diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(11): 1220-1225.

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