Protective effects of Jiangtang compound recipe on injured-steatosishepatocyte and hyper lipemia

Abstract

Abstract: AIM: To study the protective effects of Jang tang compo und recipe on injuredsteato sis hepatocyte of HepG2 cells invtiro and hyperlipemia of quails in vivo. METHODS: The hy perlipemia model of quails was induced by high fat diet in vivo and the content oftrigly ceride (TG), hig h density lipoprotein-cholesterol (HDL-C), malondialdehyde(MDA), activity of supero xide dismutase (SOD)in serums were assayed as well as patholo gical change of vascular endarterium detected. Further, the injuredsteatosis model of HepG2 cells induced with mixture fat ty acids mixed palmi tic and oleic (PA∶OA =1∶2)wast reated with vario us medicinal serum and TG, MDA, SOD activity in supernatant determined.The treated cells were al so stained with oil-redO to observe the lipid accumulation. RESULTS: The Jang tang compound recipe sig nificantly reduced intima athero scle ro tic plaque area of quails suf fered from hype rlipemia and inhibited intimal lesion. It al so inhibited lipid accumulation in HepG2 cells, reduced the content of TG, MDA and adjust the activity of SOD. CONCLUSION: Jang tang compound recipe significantly inhibits the fatty accumulation in HepG2 cells, alleviates die t-induced hyperlipidemia and the formation of atheroasclerosis in quails.

Key words: Jangtang compound recipe, HepG2, Steatosis hepatocyte, Quails

CLC Number:

R965.2

WU Guan-zhong, ZHANG Juan, HUANG Wei-jia. Protective effects of Jiangtang compound recipe on injured-steatosishepatocyte and hyper lipemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 260-265.

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