Effect of emodin on TNF-αand IL-10 gene expressions and the NF-κBactivityin the myocardial tissue after myocardial infarction in mice

Abstract

Abstract: AIM: To study the effect of emodin on TNF-and I L-10 g ene ex pressions and the NF-B activity in the myocardial tissue after myocardial infarction in mice. METHODS: The my ocardiali schemia damage model was caused by sustained left anterior descending coro nary artery ligation in mice and em odin was used to intervene with. The mRNA and protein expression levels of TNF-αand IL-10 in my ocardial tissue af teremodin intervention were detected by fluore scence quanti tative RT-PCR and ELISA.The activity of NF-κB in myocardial tissue was detected by elect rophoretic mo bility shift assay. RESULTS: After intervent ion by emodin, the expression of TNF-αwas signif icant ly reduced and the expression of IL-10 and ratios of IL-10/TNF-αwe resig nificantly increased in the my ocardial tissue of my ocardial infarction damage model. CONCLUSION: Emodinsig nificantly inhibits the expression of pro-inflammatory cytokines and the activity of NF-κB in myocardial tissue.

Key words: Emodin, NF-κB, My ocardial ischemia

CLC Number:

R965.1

WU Yan-xia, HUANG Hao, FU Lei. Effect of emodin on TNF-αand IL-10 gene expressions and the NF-κBactivityin the myocardial tissue after myocardial infarction in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 287-291.

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