Scutellarin attenuates hydrogen peroxide-induced apoptosis in PC12 cells

Abstract

Abstract: AIM: To investigate the mechanisms of inhibition of hydrogen peroxide (H2O2)-induced cell apoptosis in PC12 cells by scutellarin. METHODS: Exposure of cells to hydrogen peroxide (H2O2), a inducer of oxidative stress, triggered a typical apoptosis determined by PI singel staining, Annexin V-PI double staining and DNA agarose gel electrophoresis.Bcl-2 mRNA expression was determined by RT-PCR and caspase-3 was detected by fluoremetry. RESULTS: Scutellarin significantly inhibited H2O2-induced the DNA fragmentation and the externalization of phosphatidylserine(PS), and decreased the percentage of cell apoptosis induced by H2O2 in PC12 cells.Also, down-regulation of Bcl-2 expression and up-regulation of caspase-3 activity resulting from H2O2 exposure were significantly reduced by scutellarin in PC12 cells. CONCLUSION: Scutellarin could significantly protect cell against H2O2-induced apoptosis, which might be associated with the increase of Bcl-2 expression and the inhibition of caspase-3 activity.

Key words: scutellarin, PC12 cells, hydrogen peroxide, apoptosis, Bcl-2, caspase-3

CLC Number:

R965.2

LIU Ping, HONG Hao. Scutellarin attenuates hydrogen peroxide-induced apoptosis in PC12 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(3): 283-287.

References

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