Anti-tumor and antioxidation effects of 4-p-amino-benzoinc acid-4’-demethylepipodophyllotoxin ester

Abstract

Abstract: AIM: To investigate the antitumor and antioxidation effects of 4-p-amino-benzoinc acid-4'-demethylepipodophyllotoxin ester (PDE). METHODS: The in vitro antitumor effects was measured by MTT method, and in vivo antitumor effects was studied by transplanting tumor model of S180 and H22 in mice.The malondialdehyde (MDA) generation in tissues of rats was measured with the thiobarbituric acid (TBA) reaction.H2O2-induced RBC hemolysis was determined with spectrophotometry. RESULTS: PDE strongly inhibited SGC-7901 cell proliferation for 48 h in a concentration-dependent manner with IC50 of 84.7 (51.7-138.9) mg L.In vivo experiments showed that10, 20 mg kg PDE obviously inhibited the growth of S180 and H22 with inhibition rate 25.2%, 46.5%and 22.9%,39.3%, respectively.PDE also significantly inhibited MDA generation spontaneously [IC50 22.7 (16.9-30.4) mg L] or induced by Fe2 +-AA in homogenate of heart, liver and kidneys of rats with IC50 30.3(13.9-66.1), 29.9(20.9-42.7) and 13.3 (1.8-96.9) mg L, respectively.PDE 40, 80 mg L significantly inhibited haemolysis induced by H2O2 with inhibition rate 26.8% and 100.2%, respectively. CONCLUSION: PDE exhibits antitumor and antioxidation effects and there is relationship between the effects.

Key words: 4-p-amino-phenol-4'-denmethylepidophyllotoxin ester, antioxidation, antitumor

CLC Number:

R965.2

CHEN Xiao, WANG Zhe-yuan, ZHANG You-cheng, LI Wen-guang. Anti-tumor and antioxidation effects of 4-p-amino-benzoinc acid-4’-demethylepipodophyllotoxin ester[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(3): 288-292.

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