Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H2O2

Abstract

Abstract: AIM: To study the synergistic protective effect of picroside Ⅱ and NGF for the oxidative stress on PC12 cells induced by hydrogen peroxide (H₂O₂). METHODS: The fluorescent probe 6-carboxy-2', 7' -dichlorodihydrofluorescein (CDCFH) was used to assess the intracellular reactive oxygen species (ROS) level, and MTT assay, morphological observation as well as lactate dehydrogenase (LDH) leakage were conducted to measure cellular injury. RESULTS: The H₂O₂-induced cytotoxicity was significantly attenuated in the presence of picroside Ⅱ (25 μg/mL) and NGF (2 ng/mL).Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside Ⅱ or NGF alone-treated cells.Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects including reversing morphological changes, enhancing the ability of cell proliferation and ROS scavenging. CONCLUSION: Such action supports the therapeutic potential of picroside Ⅱ and NGF in treating nervous disorders based on their synergistic effect.

Key words: hydrogen peroxide, nerve growth factor, picrosideⅡ, reactive oxygen species

CLC Number:

R965.2

ZHANG Xiao-dong, LIU Jian-wen, LI Ting, CAO Yan, GUO Ming-chuan. Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H₂O₂[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(1): 32-37.

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Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H₂O₂

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