Protective effects of Butylphthalide on S-nitrosoglutathione-induced brain microvascular endothelial cells injury and potential mechanisms

doi:10.12092/j.issn.1009-2501.2019.05.006

Abstract

Abstract:

AIM: To investigate the protection of Butylphthalide (dL-NBP) on oxidative damage in brain microvascular endothelial cells, and to further find the potential mechanisms of dL-NBP effects. METHODS: The brain microvascular endothelial cells were injured by GSNO. The cell viability was measured by MTT assay. ROS was observed by DCFH staining. ERK, p-ERK，cleaved caspase 9，pro-caspase 9，cleaved caspase 3 and pro-caspase 3 were detected by Western blot. The gene expressions of GR, SGK and MKP-1 were detected by RT-PCR assay. RESULTS:dL-NBP (10,20 μmol/L) protected endothelial cells against GSNO-induced injury and ROS release. dL-NBP increased SGK and MKP-1 gene expression. dL-NBP up-regulated ERK phosphorylation. dL-NBP inhibited cleaved caspase 9 and cleaved caspase 3. CONCLUSION: dL-NBP attunates bEnd.3 cells GSNO-induced injury, and its mechanism is related with activating GR relative SGK and MKP-1. dL-NBP increases ERK phosphorylation and down-regulates caspase decades.

Key words: Butylphthalide, brain microvascular endothelial cells, S-nitrosyglutathione, ERK phosphorylation, reactive oxygen species

CLC Number:

R743
R966

YAN Jieping，ZHANG Shuijun，ZHANG Guobing，HU Ying，LUO Dan，JIANG Hongjuan，LI Minjing. Protective effects of Butylphthalide on S-nitrosoglutathione-induced brain microvascular endothelial cells injury and potential mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 517-522.

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