Effects of N-acetylcysteine on expression of leptin and type Ⅰ collangen in cultured rat hepatic stellate cells

Abstract

Abstract: AIM: To study the effects of N-acetylcysteine on the proliferation of rat hepatic stellate cells (HSCs) and the expression variation of leptin and type Ⅰ collagen. METHODS: HSCs were seeded at a density of 1×10⁵ cells mL per well in 96-well plates, which were grouped as follows:NAC Ⅰ, Ⅱ, Ⅲ groups(the concentration of NAC in incubation was 10, 20, 40 mmol/L respectively);Normal saline (NS) was added in the control group.The incubation liquid was extracted after cultured 48 h and was frozen at-20 ℃.Leptin and the synthesis of type Ⅰ collagen were measured by enzyme linked immunosorbent assay(ELISA) and the HSC proliferation was measured by MTT assay. RESULTS: The average absorbance of NAC Ⅲ was signficantly lower than that of the control group(P<0.05).The average type Ⅰ collagen in each NAC group was significantly lower that of the control's(P<0.01). CONCLUSION: HSC proliferation is decreased and the secretion of leptin and type Ⅰ collagen is inhibited after NAC pretreatment.

Key words: hepatic stellate cell, N-acetylcysteine, leptin, type Ⅰ collagen

CLC Number:

R965.2

CHEN Yi-ping, WANG Hong-jiao, XIU Zhi-wei, CHEN Jun-ya, SHI Hai-fan, DI Jun-bo, LI Su-hua. Effects of N-acetylcysteine on expression of leptin and type Ⅰ collangen in cultured rat hepatic stellate cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(8): 920-922.

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