Study on mechanism of changes of catecholamine content in insulin-resistance rats

Abstract

Abstract: AIM: To observe the changes of catecholamine content in the center and periphery of insulin-resistance rats induced by high-fat diet. METHODS: The formation of insulin-resistance model was assessed with serum glucose, serum insulin, index of insulin sensitivity and index of insulin resistance.The role of mifepristone antagonizing glucocorticoid was checked by the change of serum corticosterone.The blood pressure was measured by the tail cuff method. Activities of the catecholamine sympathetic nervous in the center and periphery of insulin-resistance rats were investigated by the measurements of dopamine, adrenaline, noradrenaline in hypothalamus, brainstem, serum and adrenals. RESULTS: Administration of 8 weeks of high-fat diet induced insulin-resistance rats model with increase of systolic blood pressure and catecholamine contents in the center and periphery (P<0.01).Mifepristone significantly improved blood pressure and catecholamine in the center and periphery (P<0.05). CONCLUSION: Increased catecholamine contents in the center and periphery might be related to the changes of glucocorticoid in serum of insulin-resistance rats induced by high-fat diet.

Key words: insulin-resistance, hypertension, catecholamine, sympathetic nervous, mifepristone

CLC Number:

R587

LI Shan, DING Qi-long. Study on mechanism of changes of catecholamine content in insulin-resistance rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 250-255.

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