Abstract: AIM: To design and synthesize the therapeutic vaccine against human cervical cancer and study its immunological effect.METHODS: The peptides were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Their immunological effects inducing(CTL) response were investigated in HLA-A2 transgenic mice, and the activity of specific CTL was measured by using a standard 4 h⁵¹Cr release assay.RESULTS: The purities of peptides were 86.5 %, 81.8 % and 98.5 %, respectively.Their molecular weights were 3 121, 2 580 and 815 respectively, which was the same as expected molecular mass.The therapeutic vaccine could induce strong specific CTL activity in HLA-A2 transgenic mice.CONCLUSION: The synthetical products are the target peptides.The combination of CTL and T helper epitope and lipid molecule can remarkably improve the immunogenicity of CTL peptide.

Key words: cervical cancer, epitope, therapeutic vaccine, solid phase synthesis