Differentiation-inducing activity of tubeimoside I in human erythroleukemicK562 cells

Abstract

Abstract: AIM: To examine the differentiation-inducing effect of tubeimoside I on human erythroleukemic K562 cells.METHODS: The growth inhibitory activity of tubeimoside I was evaluated with K562 cells.Trypan blue exclusion , morphological observation , cytochemistry were used to observe the features of erythroid , granulocytic,and megakaryocytic lineages differentiated from K562 cells.Using Western blot analysis , we studied alterations in c-myc and c-fos expression accompanying tubeimoside I-induced differentiation of K562 cells.RESULTS: Tubeimoside I inhibited the proliferation K562 cells and induced the differentiation of K562 cells into the erythrocyts.It could down-regulate c-myc and up-regulate c-fos ,and the steps were found to be the early events of K562 cell differentiation mediated by tubeimoside I.CONCLUSION: Tubeimoside I can induce the differentiation of K562 cells into the erythrocytes , and down-regulation of c-myc and up-regulation of c-fos which are the early responses of K562 cells to the induction of differentiation mediated by tubeimoside I.

Key words: tubeimoside I, differentiation-inducing activity, K562 cells

CLC Number:

LIU Ji-yan, GAO Ying, YU Li-jian, MA Run-di. Differentiation-inducing activity of tubeimoside I in human erythroleukemicK562 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 743-747.

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