Effect of recombinant human endostatin on expression of vascular endothelial cell growth factor in adjuvant arthritis synovium tissue

Abstract

Abstract: AIM: To investigate the effect of recombinant human endostatin on the expression of vascular endothelial cell growth factor (VEGF) in adjuvant arthritis synovium tissue.METHODS: Adjuvant arthritis rat model was induced by a single intradermal injection of 100 μl of Freund' s complete adjuvant(CFA) on d 0.The volume of rat hind paw was measured by means of Volume Meter.The expression of VEGF and microvessel density of the synovial tissue were measured by immunohistochemical method.RESULTS: The secondary inflammation of Adjuvant-induced arthritis(AA) rats appeared on the d 10 after the injection of CFA.The therapeutic administration of recombinant human endostatin(1.25, 2.5, 5.0 mg·kg^-1 )were given for 7 days consecutively.Methotrexate (MTX)(1.0 mg·kg^-1 )was given only once on d 10 as the control.It was found that recombinant human endostatin significantly inhibited the secondary paw swelling.Recombinant human endostatin significantly decreased the expression of VEGF and microvessel density of the synovial tissue on AA rats.CONCLUSION: Recombinant human endostatin had an inhibitory effect on the expression of VEGF and microvessel density of the synovial tissue on AA rats, which was related to its anti-angiogenesis and an inhibition of VEGF on synoviocytes.

Key words: recombinant human endostatin, adjuvantarthritis, vascular endothelial cell growth factor, microvessel density

CLC Number:

R965
R966

XIA Li-juan, CHEN Fei-hu, HUANG Xue-ying, LIU Yong-jing, LI Jun. Effect of recombinant human endostatin on expression of vascular endothelial cell growth factor in adjuvant arthritis synovium tissue[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 760-763.

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