Inhibitory effects of tubeimoside 1, a cyclic bisdesmoside isolated from Bolbostemma paniculatum, on metastases of mouse B16 melanoma and lewis lung carcinoma

Abstract

Abstract: AIM: To investigate the effects of tubeimoside 1 on the metastases of mouse B16 melanoma and lewis lung carcinoma.METHODS: The effects of tubeimoside 1 on metastasis were studied by using an experimental metastasis model of mouse B16 melanoma cell line and a spontaneous metastasis model of mouse lewis lung carcinoma.The effects of tubeimoside 1 on the expressions of CD44v6, ErbB-2, and nm23-H1 were studied by using streptavidin-peroxidase two step immunohistochemical method.RESULTS: In mice that received i.v.injec-tion of mouse B16 melanoma cells, daily i.p.administration of tubeimoside 1-without reaching systemic toxicic levels-remakably reduced lung weight, and number of lung metastases, with preservation of apparently healthy and active behaviors.Lung metastasis inhibition rates of B16 melanoma cells by tubeimoside 1 (2, 3 mg·kg^-1·d^-1×14 d) and cyclophosphamide (50 mg·kg^-1 ·wk^-1 ×2d) were 68.8 %, 82.8 %, and 49.1 %, respectively.The weight of primary tumors of mouse lewis lung carcinomain the tubeimoside 1-administered mice was significantly less than that of the control (P<0.05).The liver metastasis inhibition rates of lewis lung carcinoma by tubeimoside1 (2, 3 mg·kg^-1·d^-1 ×14 d) and cyclophosphamide(50 mg·kg^-1 ·w^-1 × 2 d ) were 46.3 %,52.0 %, and 54.7, respectively.Treatment of tubeimoside1 apparently resulted in down-regulation of expressions of CD44v6 and ErbB-2, and up-regulation of expression of nm23-H1 in mouse lewis lung carcinoma cells.CONCLUSION: These observations qualify tubeimoside 1 as an interesting lead compound to prevent cancer spread and metastatic growth.

Key words: Bolbostemma paniculatum, tubeimoside1, cancer metastasis, mouse B16 melanoma, mouse lewis lung carcinoma, metastasis relnted gene

CLC Number:

WANG Chang-xiu, MA Run-di, YU Li-jian. Inhibitory effects of tubeimoside 1, a cyclic bisdesmoside isolated from Bolbostemma paniculatum, on metastases of mouse B16 melanoma and lewis lung carcinoma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 764-770.

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