Abstract: AIM: To analyse the intestinal absorbable compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy (ILC ).METHODS: The ILC column separation and retention of the extracts of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae extracted with different solvents were observed.Also analysed were the contents of quercetin and apigenin in 75 % ethanol extract of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae.RESULTS: It was found that the 75 % ethanol extract was better than the water extract based on the number and peak areas of retention peaks.In the 75 % ethanol extract of Erzhi Pills, the content of quercetin was 9.6 μg·g^-1 , and the content of the apigenin was 2.7 μg·g^-1.The content of quercetin and apigenin in the 75 % ethanol extract of Herba Ecliptae corresponded to 2.8 and 3.8 μg·g^-1 respectivelly.In the 75 % ethanol extract of Fructus Ligustri Lucidi, the content of quercetin was 9.1 μg·g-1 , but apigenin was not detected.CONCLUSION: Quercetin and apigenin are two main retained components of Erzhi Pills on ILC, the former is contributed by both Fructus Ligustri Lucidi and Herba Ecliptae, the latter only by Herba Ecliptae.Quercetin and apigenin were predicted to be intestinal absorbable components of Erzhi Pills with ILC.Immobilized liposome chromatagraphy can be used as a tool in screening possible bioactive ingredients and in quality control of traditional Chinese medicine (TCM).

Key words: Immobilized liposome chromatagraphy, Erzhi Pills, Fructus Ligustri Lucidi, Herba Ecliptae, main bioactive ingredients