Study on absorption of ginsenoside Rg1 using rat ansa intestinal model

Abstract

Abstract: AIM: To develop an accurate, sensitive and reliable HPLC-MS method for the determination of ginsenoside Rg1 in rat plasma, and to study the absorption characteristics of Rg1 at different intestinal sections (duodenum, ileumand, jejunum and colon ) of rats.METHODS: Ginsenoside Rg1 or total ginsenoside which containing equal amount of Rg1 was injected into different intestinal sections (duodenum, ileum, jejunum and colon) of each rat.At the same time, Ginsenoside Rg1 or total ginsenoside was administrated to rats by intragastric gavage.Blood samples were collected according to preset timetable and Rg1 concentrations were determined.RESULTS: The bioavailability of Rg1 in rats was increased significantly when total ginsenoside was administrated compared with that of Rg1 given alone.The absorption profile of Rg1 was different in duodenum and ileum but similar in jejunum and colon.CONCLUSION: There might be some specific substance in total ginsenoside,which could improve the absorption of Rg1 in duodenum and ileum.

Key words: total ginsenoside, ginsenoside Rg1, ansaintestinal model, absorption, HPLC-MS

CLC Number:

LV Tian, SUN Jian-guo, LV Hua, XU Mei-juan, XIE Hai-tang, LI Hao, WANG Guang-ji. Study on absorption of ginsenoside Rg1 using rat ansa intestinal model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 780-783.

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Study on absorption of ginsenoside Rg1 using rat ansa intestinal model

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