Investigation of rat osteoporosis resulting from hyperlipidemia

Abstract

Abstract: AIM: To establish the hyperlipemic rat model by long-term gastric perfusion of intralipid and investigate the effects of hyperlipidemia on bone.METHODS: Eighteen SD rats were randomly divided into control group (C) and high-fat-diet group (HDF), with 9 rats per group.Rats of C group were treated orally with normal saline, and rats in HDF group were oral gavage of intralipid at dose of 5.0 mL/kg, once a day for 20 weeks. At the end of experiment, their serum levels of lipid were measured.Bone histomorphometric analysis of thighbone was performed in undecalcified sections, and the length and width of ulnar were measured.RESULTS: Compared with the C group, serum TC and low density lipoproteins (LDL) were increased (P <0.01) and high density lipoproteins (HDL) were decreased (P <0.01). Bonehistomorphometric parameters showed a significant bone loss in the model group (P <0.01).CONCLUSION: Bone loss occurs in the hyperlipemic rat model by long term gastric perfusion of intralipid.

Key words: intralipid, hyperlipidemia, osteoporosis, rats

CLC Number:

R965.2

YOU Ting-ting, WU Tie, ZHANG Zhi-ping, ZOU Li-yi, HUANG Bo. Investigation of rat osteoporosis resulting from hyperlipidemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(3): 299-303.

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