Protective effect of insulin-like growth factor-1 on apoptosis in murine pancreatic β-cells

Abstract

Abstract: AIM:To investigate the protective effect of insulin-like growth factor-1 (IGF-1)on apoptosis in murine pancreatic β-cellsMIN6 induced by tert-butyl hydroperoxide(tBHP).METHODS: MIN6 cells were cultured and divided into four groups: control group, tBHP (25 μmol/L)group, IGF-1(100 ng/mL)group and IGF-1 +tBHP group. Cell damage was detected by AO-EB staining and TUNEL assay. The levels of nitric oxidewere measured by Griess assay.RESULTS:Induced cell apoptosis and increased NO production were observed when MIN6 cells treated with 25 μmol/L of tBHP for 1 h, When the cells were pretreated with 100 ng/mL IGF-1 for 24 h before tBHP exposured, cell apoptosis induced by tBHP was significantly inhibited and NO production was also decreased.CONCLUSION:The present study suggests that IGF-1 has protective effect on apoptosis in murine pancreatic β-cells MIN6 induced by tBHP. The mechanism of this effect is possibly attributed to IGF-1 reduces NO production which induced by tBHP.

Key words: insulin-like growth factor-1, pancreatic β-cell, tert-butyl hydroperoxide, apoptosis, protection

CLC Number:

R965. 2

LIU Xiao-hong, CHEN Zhou, WANG Yan-ping, LIU Li-bin. Protective effect of insulin-like growth factor-1 on apoptosis in murine pancreatic β-cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(6): 645-648.

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