Effects of recombinant human endostatin on the expression of apoptosisrelated genes in synovial tissue in rats with adjuvant arthritis

Abstract

Abstract: AIM: To observe the effects of recombinant human endostatin (rh-end) on the expression of p53, fas and bcl-2 genes in synovial tissue in rats with adjuvant arthritis (AA). METHODS: The effects of rhend on the expression of p53, fas and bcl-2 mRNA in synovial tissue in AA rats were examined quantitatively by SYBR Green I real-time fluor, cent quantitative PCR (FQ-PCR). RESULTS: The expression of fas and bcl-2 mRNA in synovial tissue inprats treated with rh-end was significantly increased, as compared with model con-trols (P < 0.01). However, p53 mRNA expression decreased significantly in rh-end group than that in control (P < 0.01). CONCLUSION: Recombinant human endostatin may induce fas-mediated apoptosis of synovial cells in AA rats, which may be independent on p53 and bcl-2.

Key words: recombinant human endostatin, adjuvant arthritis, synovial cell, apoptosis-related gene

CLC Number:

R965.2

HUANG Xue-ying, CHEN Fei-hu, LIU Yong-jing, ZHANG Xiao-ming, YUAN Feng-lai. Effects of recombinant human endostatin on the expression of apoptosisrelated genes in synovial tissue in rats with adjuvant arthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(12): 1359-1363.

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