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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (7): 755-759.

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Inhibition of NF-kappa B activation by Bay117082 attenuate apoptosis induced by HCPT in Breast cancer Bcap 37 cells

YE Meng, LIN Lei, FANG Yong1, PAN Hong-ming1, WU Jin-min1   

  1. Department of Oncology, the Affiliated Hospital, Ningbo University, Ningbo 315020, Zhejiang, China; 1Department of Oncology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, Zhejiang, China
  • Received:2005-01-05 Revised:2005-03-30 Online:2005-07-26 Published:2020-11-10

Abstract: AIM: To investigate the role of Bay117082 in apoptosis induced by 10-Hydroxycamptothecin (HCPT) in human breast carcinoma cells. METHODS: The cell growth inhibiting was measured by MTT assay, agarose gel eletrophoresis were performed for cell apoptosis, Western Blotting were performed for protein expression. DIG-EMSA was conducted to determine the DNA-binding activation of NF κB. RESULTS: 5 mmol·L-1 Bay117082 had a remarkable inhibition on apoptosis induced by HCPT in breast cancer cells. NF κB was not activated after exposing to HCPT plus Bay117082.Pro-Caspase3 and Bcl-2 were not degraded either. CONCLUSION: Bay117082 may attenuate apoptosis induced by HCPT through inhibiting activation of NF κB in Bcap37 cell line.

Key words: HCPT, breast carcinoma cell, apoptosis, Bay117082, NFκB

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