Protective effect of angiotensin-(1-7) on human umbilical vein endothelial cells injury induced by angiotensin II in culture

Abstract

Abstract: AIM: To investigate the protective effects of angiotensin-(1-7) [Ang-(1-7)] on human umbilical vein endothelial cells (HUVECs) injury induced by angiotensin II (Ang II) in culture.METHODS: Cultured human umbilical vein endothelial cells were randomly divided into 4 groups:control, Ang II, Ang-(1-7), and AngII+Ang-(1-7).Spectrophotometer and flow cytometry were used to evaluate lactate dehydrogenase (LDH) leakage content and apoptosis percentage, respectively. Nitric oxide (NO) and endothelin-1 (ET-1) contents were measured by colorimetry and radioimmunoassay.RESULTS: 0.1 μmol°L^-1 Ang II significantly increased LDH leakage (P<0.01), ET-1 release (P<0.01), and apoptosis percentage (P<0.01).These increases were inhibited by Ang-(1-7) in a dose-dependent manner, while NO release by HUVECs was promoted by Ang-(1-7).Ang-(1-7) alone had no effect on HUVECs. CONCLUSION: Ang-(1-7) has a protective effect on HUVECs via inhibition of Ang II-induced injury and apoptosis, suggesting that Ang-(1-7) may play an important role in prevention and treatment of vascular diseases.

Key words: angiotensin-(1-7), angiotensin II, human umbilical vein endothelial cells, apoptosis, nitric oxide, lactate dehydrogenase, endothelin

CLC Number:

R965.1

ZOU Jun, WANG Hong, FENG Dan. Protective effect of angiotensin-(1-7) on human umbilical vein endothelial cells injury induced by angiotensin II in culture[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(2): 180-183.

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