Abstract: AIM: To study the pharmacokinetics and absolute bioavailability of breviscapine in mice.METHODS: After iv injection (50 mg°kg^-1)and ig administration (150 mg°kg^-1)of breviscapine in mice, the plasma scutellarin concentration were detected by SPE-HPLC method.The pharmacokinetic parameters were calculated by 3p97 program.RESULTS: The plasma scutellarin concentration-time curve after iv injection of breviscapine was fitted with a three compartment model.AUC, C₀ and T_1/2β were 12.97 mg°h°L^-1, 132.23 mg°L^-1 and 4.04 h, respectively.After ig administration of breviscapine, the drug absorption was rapid, but the plasma concentration was very low.The parameters of AUC and T_1/2Ke calculated with non-compartment model were 1.966 mg°h°L^-1 and 3.4 h, respectively.The absolute bioavailability was 5.05%.CONCLUSSION:The pharmacokinetics of breviscapine in mice after iv injection fitted with three-compartment model.After ig administration, the drug is absorbed rapidly but the absolute bioavailability is very low, and the changes of plasma concentration of scutellarin is not regulation.

Key words: breviscapine, scutellarin, mice, pharmacokinetics, bioavailability