Abstract: AIM: To isolate and extract anti-endotox effective components in Radix Paeoniae Rubra.METHODS: By the biosensor technology, the anti-endotoxin effective components were isolated and extracted with general separate technology of Chinese traditional medicine. Through quantificational mensuration for LPS and inhibition of TNF-αrelease from endotoxin-stimulated cells in vitro, anti-endotoxin activity of Radix Paeoniae Rubra were elucidated.RESULTS: The effective components had high binding capability with LPS.After incubation with endotoxin and cells, the capability showed anti-endotoxin and inhibition of TNF-αrelease from endotoxinstimulated cells in vitro.CONCLUSION: The anti-endotoxin effective components isolated and extracted using LPS as target by the biosensor technology are available in searching anti-endotoxin agents from Radix Paeoniae Rubra.It shows that Radix Paeoniae Rubra has high ability on neutralization with LPS.

Key words: Radix Paeoniae Rubra, biosensor technology, endotoxin,extraction, isolation