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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (3): 348-351.

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Preparation of liposomes encapsulating dermatophagoides farinae antigen and it's influence on immunity of mites sensitized mice

YUE Wen-yan, ZHOU Shun-lin, LIU Wen-yan, WANG Shao-sheng, CHEN wen-kui   

  1. Department of Parasitology, Wannan Medical College, Wuhu 241001, Anhui, China
  • Received:2005-01-05 Revised:2005-03-08 Online:2005-03-26 Published:2020-11-18

Abstract: AIM: To investigate the changes on immunological function in antigen-sensitized mice injected by dermatophagoides farinaeliposomes (DF) subcutaneously.METHODS: BALB/c mice were sensitized with DF antigen injected subcutaneously.After injected by DF liposomes with 1, 3, and 9 μg°g-1 dosage respectively, the total IgE, total IgG, DF specific IgE(sIgE), DF specific IgG (sIgG), IL-4, and IFN-γwere detected by ELISA.RESULTS: The total IgE, sIgE, total IgG, and sIgG in the serum of sensitized mice increased, the level of IFN-γreduced and IL-4 increased.After injected subcutaneously by DF liposomes, the total IgE, sIgE and IL- 4 in the serum of mice of all the treatment groups reduced, and IFN-γraised, and the total IgG, sIgG raised in the serum of mice administrated with small andmedium dosage, but the low dosage was more obvious.CONCLUSION: DF liposomes have some effects on the immunological function in mite-sensitized mice in the low dosage.

Key words: dermatophagoides farinae, liposome, specific IgE, specific IgG, total IgE, total IgG

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