Abstract: AIM: To develop a method for determi-nation of the biological activity of atranticance. METHODS: The conditions of the experiment were determined by orthogonal test, then IC 50 which was the concentration of the sample when the 50% death of cells was tested. RESULTS: The mortality rate had a good correlativity with Log consistency of the sample, (r =0.9515, P<0.01). And the coefficients of variation of reproducibility of intra-, inter and different test assays were 12.3%, 16.6% and 17. 8%, respectively. CONCLUSION: Thismethod is superfine, simple and highly reproducible. It can be used for determining the biological activity of a-tranticance.

Key words: atranticance, activity, orthogonal test, MTT, K562