Effects of fluvastatin on expression of intercellular adhesion molecule-1 after myocardial ischemia-reperfusion in rabbits with hyperlipidemia

Abstract

Abstract: AIM: To investigate the effects of fluvas-tatin on expression of intercellular adhesion molecule-1 af-ter myocardial ischemia-reperfusion in rabbits with hyper-lipidemia. METHODS: 30 rabbits which were gastric perfusion administered intralipid were randomly divided into 3 groups (n=10 in each); IR (ischemia-repeifu-sion) group, S (sham-operation) group and F (fluvastatin 10 mg·kg^-1) group. Electrocardiography and cardiac function were recorded during the experiment. At the end of reperfusion, ischemic area and infarct size were defined by Evans blue and triphenyltetrazolium chloride staining. The expression of ICAM-1 in myocardium was measured by RT-PCR. RESULTS: After ischemia-reperfusion, the expression of ICAM-1 mRNA in myocardial and infarct size decreased and cardiac function significantly improved in F group compared with IR group. CONCLUSION: The increase of expression of ICAM-1 mRNA in myocardi-al may be one of the important factors in inducing myocar-dial ischemia-reperfusion injury. The myocardial protec-tive mechanism of fluvastatin maybe attribute to its effect on decreasing the expression of ICAM-1 mRNA in myo-cardial.

Key words: fluvastatin, myocardial ischemia-reper-fusion, intercellular adhesion molecule-1, hyperlipidemia

CLC Number:

R972.6

QIN Jin-Mei, YANG Bo, LI Jian-Jun, CHEN Li, DAI Shu-Hua. Effects of fluvastatin on expression of intercellular adhesion molecule-1 after myocardial ischemia-reperfusion in rabbits with hyperlipidemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(11): 1281-1284.

References

1 马建林,毛焕元.自制脂肪乳剂灌胃在家兔高脂血症模型制作中的应用[J].同济医科大学学报,1999;28(5):409-11
2 Kaminski KA, Bonda TA, Korecki J, Musial WJ. Oxidative stress and neutrophil activation-the two keystones of ischemia/ repeifusion injury[J]. Int J Cardiol, 2002; 86(1): 41-59
3 Sun B, Fan H, Honda T, Fujimaki R, Lafond-Walde A, Masui Y, et al. Activation of NF kappa B and expression of ICAM-1 in ischemic-reperfused canine myocardium[J]. J Mol Cell Car-diol, 2001;33(1):109-19
4 Ikeda U, Ikeda M, Kano S, Shimada K. Neutrophil adherence to rat cardiac myocyte by proinflammatory cytokines[J]. JCard-iovasc Phannacol, 1994; 23 (4): 647-852
5 Kacimi R, Karliner JS, Koudssi F, Long CS. Expression and regulation of adhesion molecules in cardiac cells by cytokines: response to acute hypoxia[J]. Circ Res, 1998;82(5):576-86
6 Youker KA, Hawkins HK, Kukielka GL, Perrard JL, Michael LH, Ballantyne CM, et al. Molecular evidence for induction of intracellular adhesion molecule-1 in the viable border zone assi-ciated with ischemia-reperfusion injury of the dog heart[J]. Circulation, 1994;89(6):2736-46
7 Metzler B, MairJ, LercherA, SchaberC, HintringerF, Pach-inger O, et al. Mouse model of myocardial remodelling after ischemia: role of intercellular adhesion molecule-1 [J]. Cardio-vasc Res, 2001; 49 (2): 399-407
8 Hartman JC, Anderson DC, Wiltse AL, Lane CL, Rosenbloom CL, Manning AM, et al. Protection of ischemic/reperfused ca-nine myocardium by CL18/6, a monoclonal antibody to adhesion molecule ICAM-1 [J]. Cardiovasc Res, 1995; 30(1): 47-54
9 Yokota N, O'Donnell M, Daniels F, Bume-Taney M, Keane W, Kasiske B, et al. Protective effect of HMG-CoA reductase inhibitor on experiment renal ischemia-repeifusion injury [J], Am J Nephrol, 2003;23(1): 13-7
10 Gueler F, Fong S, Prak JK, Fiebeler A, Menne J, Eiger M, et al. Postischemic acute renal failure is reduced by short-tenn statin treatment in a rat model[J]. JAM Soc Nephrol, 2002; 13 (9):2288-98
11 Ikeda Y,Young LH,Lefer AM. Rosuvastatin,a new HMG-CoA reductase inhibitou, protects ischemic reperfused myocar-dium in normocholesterolemic rats[J]. J Cardiovasc Pharmacol, 2003;41(4):649-56
12 Wayman NS, Hattori Y, Mcdonald MC, Mota-Filipe H, Cuzzo-crea S, Pisano B, et al. Ligands of the peroxisome proliferator-activated receptors (PPAR-gamma and PPAR-alpha) reduce myocardial infarct size[J]. FASEB J, 2002; 16(9): 1027-40

[1]	ZHAO Lu, LI Fuyong, WEN Binhong, LI Daowei. Effects of stains on hyperlipidemia and pain relieve of patients with chronic migraine [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 671-675.
[2]	XU Ke, GAO Junwei, LIU Peng, SHEN Zhengri, JIA Hui, WU Chenguang, TIAN Feng, WANG Lifan, LI Ping. Tangshen formula improves cholesterol uptake and efflux of macrophages induced by high lipid via activating PGC-1α/LXR/ABCA1 pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(9): 978-985.
[3]	LI Jiadan，LU Chen. Synergistic effects of Cortex Mori alkaloids combined with Simvastatin in rats with hyperlipidaemia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(3): 278-284.
[4]	ZHENG Li, MO Juanfen, WU Jiayuan, GUO Li, BAO Yi. Study on the anti-hyperlipidemia mechanisms and signaling pathways of traditional Chinese medicine Polygonum cuspidatum based on network pharmacology [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1107-1119.
[5]	LI Xi, JIN Gaofeng, LIU Zhijun, LI Xinhua, LIU Mingyi, ZHANG Hong, XIONG Yuqing. Relevance of plasma distribution of microRNA-148a in Chinese population and hyperlipidemia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 659-663.
[6]	YANG Yi, GUAN Qiaobing, GUO Li, LI Wenyan, ZHANG Hui, HAN Chenyang. Alternate dosing of ezetimibe and colestipol on regulating lipid metabolism in hyperlipidemia rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(10): 1112-1116.
[7]	JIANG Hai, JIANG Guozhi, CHEN Zhong, LI Zhenjiang. Lipid-lowering effects of combined use of curcuma longa L.extracts and atorvastatin calcium in hyperlipidemia mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 48-51.
[8]	BAI Yun-xia. Study of the effects and the mechanism of resveratrol on platelet aggregation in hyperlipidemia rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(7): 769-773.
[9]	KONG Xiang, YANG Jie-ren, GUO Li-qun, ZHOU Yong. Effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 366-370.
[10]	YANG Ying, YUAN Bin, ZHOU Chun-yang, CHENG Long-xian. Effects of naringin and naringenin on the level of peroxisome proliferator-activated receptors δ in monocyte of diet-induced obese rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(1): 6-10.
[11]	LIU Jing, XIONG Yu-qing. Influence of related genetic polymorphisms to pharmacokinetics and pharmacodynamics of Fluvastatin [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(11): 1282-1288.
[12]	HUO Yong-jia. Effects of fluvastatin on exercise-induced myocardial ischemia in patients with stable exertional angina pectoris and hypercholesterolemia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 200-203.
[13]	LI Jing-yuan, CAI Jia-li, DAI Ren-ke, XIE Shui-lin, DENG Ji-feng, LU Pei-feng, LI Ren-shu, SUN He, LIU Yan-huai. Study on the relationship between pharmacokinetics and pharmacodynamics for statins in hyperlipidemia model in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(12): 1321-1325.
[14]	FAN Lei, SONG Hong-tao, XU Rong-qing, XU Zheng-wei. Correlation between Apolipoprotein CⅠ gene polymorphisms and lipid-lowering efficacy of atorvastatin [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(10): 1161-1165.
[15]	LI Li-hua, XU Hui-qin. Effects of Yinqu Capsule on blood lipid in rats with experimental hyperlipidemia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 99-102.