Effects of Fr. A in Guizhi Tang on the excretion of PGE2 and its signal transduction components in cerebral microvascular endothelial cells stimulated by IL-1

Abstract

Abstract: AIM: To investigate the effects of Fr. A, an antipyretic active fraction extracted from Guizhi Tang, on the excretion of PGE₂ and its signal transduction com-ponents in cerebral microvascular endothelial cells (rCMEC) stimulated by IL-1. METHODS: Fr. A-containing rat serums were prepared. The PGE₂, PLA₂, COX and NO were measured by radioimmunoassay, enzyme reaction substrate assay, ELISA and colorimetry, respectively. RESULTS: When administration of Fr. A-containing serum, contents of PGEj and NO, the activity total COX and of COX-2 were significantly decreased in rCMEC. But the activity of sPLA₂ was not significantly decreased. CONCLUSION: Fr. A can affect the level of PGE₂ by iniluencing the main elements of COX and NO in PGE₂ signal transduction passway.

Key words: active fraction A of Guizhi Tang, cere-bral microvascular endothelial cells, interleukin-1, pros-taglandin E₂, signal transduction

CLC Number:

R285.5

JIANG Nan, HUO Hai-Ru, Li Lan-Fang, GUO Shu-Ying, JIANG Ting-Liang. Effects of Fr. A in Guizhi Tang on the excretion of PGE₂ and its signal transduction components in cerebral microvascular endothelial cells stimulated by IL-1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(11): 1289-1292.

References

1 谭余庆,李晓芹,霍海如,郭淑英,周爱香,田甲丽,等.桂枝汤有效部位A对体温的双向调节作用及其机理研究一对大鼠下丘脑PGE₂含量的影响[J].中国实验方剂学杂志,1998; 4(2): 11-3
2 霍海如,李晓芹,谭余庆,郭淑英,周爱香,田甲丽,等.桂枝汤有效部位A对体温双向调节作用及其机理研究:对下丘脑NE、DA、5-HT含量的影响[J].中国实验方剂学杂志,1998; 4(3): 14-6
3 陈云波,何燕飞.血管内皮细胞的生物学功能[J].生物学通报,1999; 29(11):11-3
4 Inagami T, Naruse M, Richard H. Endothelium as an nedocrine organ[J]. Annu Rev Physiol, 1995; 579(3): 171-89
5 Coceani F. Further evidence implicating prostaglandin E2 in the genesis of pyrogn fever [J]. Am J Physiol Regul Integr Comp Physiol, 1988; 254(5): 463-9
6 Gordon EL, Danielsson PE, Nguyen TS, Winn HR. A compar-ison of primary cultures of rat cerebral microvascular endothelial cells to rat aortic endothelial cells [J]. In Vitro Cell Dev Biol, 1991; 27A(4); 312-26
7 Stitt JT. Evidence for the involvement of the organum vasculo-sum laminae terminals in the febrile response of rabbits and rats [J]. J Physiol (Lond), 1985; 368(11):501-11
8 Ivanov AI, Pero RS, Scheck AC, Romanovsky AA. Pros-taglandin E₂-synthesizing enzymes in fever: differential tran-scriptional regulation [J]. Am J Physiol Regul Integr Comp Physiol, 2002; 283 (5): 1104-17
9 Kudo I, Murakami M. Phospholipase A₂ enzymes [J]. Pros-taglandins Other Lipid Mediat, 2002;68-69(8):3-58
10 Balsinde J, Balboa MA, Dennis EA. Functional coupling be-tween secretory phospholipase A₂ and cyclooxygenase-2 and its regulation by cytosolic group IV phospholipase A₂ [J]. Proc Natl Acad Sci USA, 1998;95 (14): 7951-6
11 Inoue T, Fukuo K, Morimoto S, Koh E, Ogihara T. Nitric ox-ide mediates interleukin-1-induced prostaglandin E2 production by vascular smooth muscle cells [J]. Biochem Biophys Res Comrniin, 1993; 194(1): 420-4
12 Davidge ST, Baker PN, McLaughlin MK, Roberts JM. Nitric oxide produced by endothelial cells increases production of eico-sanoids through activation of prostaglandin H synthase[J]. Cir-culation Research, 1995; 77(8): 274-83

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Effects of Fr. A in Guizhi Tang on the excretion of PGE₂ and its signal transduction components in cerebral microvascular endothelial cells stimulated by IL-1

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