Abstract: AIM: To investigate the effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cytokines production of serum-free cultured normal human keratinocytes stimulated by PMA and lipopolysaccharides(LPS).METHODS: The influences of rhIL-10 on the proliferation of serum-free cultured normal human keratinocytes were assayed by MTT.Interleukin-1, interleukin-6, and interleukin-8 was detected by Enzymelinked immunosorbent assay (ELISA).RESULTS: The rhIL-10 significantly inhibited the proliferation and the production of IL-1, IL-6 and IL-8 by KC which was activation by PMA LPS with dose-dependent manner.CONCLUSION: The rhIL-10 inhibits the proliferation and cytokines production of serum-free cultured normal human keratinocytes.The anti-psoriatic effects of rhIL-10 may be related to inhibiting hyperplasia of epidermal cells, promoting the formation of granular cells and possessing combined anti-inflammatory and immunomodulatory activity.

Key words: recombinant human interleukin-10, keratinocytes, cytokines, psoriatic