DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes

Abstract

Abstract: AIM: To evaluate the inhibition effects of DNAzymes specific to Methecillin-resistant staphylococcus aureus (MRSA)drug-resistant gene blaR1 on the expressions of drug-resistance. METHODS: mRNA of blaR1 was chosen as a target gene and DNAzyme DrzB specific to it was designed and synthesized.After DrzB was introduced into the MRSA, drug-resistant characters of MRSA were evaluated by cloning efficiency.The inhibition effects of DrzB on the expressions of drug-resistant gene blaR1 were observed by RT-PCR in MRSA. RESULTS: Colony forming units (CFU)of MRSA incubated with DrzB on the M-H agar added oxa (6 mg·L^-1)were less than those of control group (P <0.01).Levels of blaR 1 mRNA of the DrzB group were lower than those of the control group (P <0.01). CONCLUSION: Antibiotic sensitivity on MRSA can be partially restored by exogenously delivered DrzB targeted to mRNA of blaR1 in blocking drug-resistant genes expression.DNAzyme DrzB is proved a specific and effective gene therapeutic means.

Key words: Methecillin-resistant staphlococcus aureus(MRSA), drug-resistance, gene expression, DNAzyme, inhibition

CLC Number:

R965.1

LIU Jie, LUO Xiao-Xing, MENG Jing-Ru, HU Ben-Quan, WANG Hai-Fang, MENG Jia. DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(6): 641-645.

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