Abstract: AIM: To develop a method for the determination of trans-resveratrol in rat plasma. METHODS: Plasma samples were performed on a reversed-phase column by gradient elution after liquid-liquid extraction. RESULTS: Good linearity (r=0.9998) was obtained in the range of 0.0035-1.400 mg·L^-1 for trans-resveratrol, and the limit of quantitation (LOQ) was 0.035 mg·L^-1.The average recovery was 103.0 %and the RSD of intra-day and inter-day was less than 10 %.This method was applied to the determination of resveratrol in rat plasma and the pharmacokinetical parameters were calculated as follows:t1 2=11.5 min, t_max=10.0 min, and C_max=1.93 mg·L^-1. CONCLUSION: A reliable RPHPLC method for resveratol determination is developed, and appropriate for the determination of resveratrol in rat plasma.

Key words: resveratrol, Polygonum cuspidatum extractant, RP-HPLC, pharmacokinetics