Reversal of multidrug resistance by CPUE1 in K562 A02 cells

Abstract

Abstract: AIM: To study the effect of CPUE1 on the reversal of multidrug resistance (MDR) in K562/A02 cells and its mechanism. METHODS: MTT assay was used to determine the influence of CPUE1 on the cytotoxicity of vincrisine (VCR) in K562/A02 cells.The effect of CPUE1 on the apoptosis induced by VCR in K562/A02 cells was detected using DNA analysis and Annexin-Ⅴ PI double stain assay.Flow cytometry was used to investigate the efflux of rhodamine123 (Rh123) by P-glycoprotein (P-gp) in K562 /A02 cells. RESULTS: CPUE1 increased the cytotoxicity and apoptosis induced by VCR in K562 A02 cells.At the concentration of 10 μmol·L^-1, CPUE1 reduced the IC50 value of VCR from 60.54 to 4.17 μmol·L^-1.CPUE1 inhibited the efflux of Rh123 and resulted in the increase of intracellular accumulation of Rh123 in K562/A02 cells. CONCLUSION: CPUE1 has strong reversal effect on MDR in K562/A02 cells by inhibiting P-gp activity.

Key words: CPUE1, vincristine, P-glycoprotein, apoptosis, multidrug resistance

CLC Number:

R966

ZHU Hao-Jie, HOU Yuan-Yuan, LIU Guo-Qing. Reversal of multidrug resistance by CPUE1 in K562 A02 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(5): 544-547.

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