Effects of EGb on apoptosis and NOS activity in rat hippocampal neurons induced by hypoxia

Abstract

Abstract: AIM: To observe the effects of EGb (Ginkgo biloba extract) and Gin(Ginkgolide) on rat hippocampal neurons induced by H/R(Hypoxia/Reoxigen) with regards of apoptosis and NOS activity.METHODS: Primarily cultured E 15 day-fetal rat hippocampal neurons were used to establish H/R injury model including five groups:Normal group, H/R group, Drug group (EGb group and Gin group) and Positive control group (MK-801 group).The neuronal apoptosis were measured by TUNEL staining.The NOS activity was determined by NADPH-d histochemical analysis.RESULTS: The apoptosis cell numbers of EGb and Gin groups were lower than those of H/R group.The preincubation of EGb and Gin decreased NADPH-positive cells.CONCLUSION: EGb and Gin protects rat hippocampal neurons induced by H/R from apoptosis and suppressed NOS activity.

Key words: primary culture, hippocampal neuron, hypoxia, EGb, apoptosis, NOS

CLC Number:

R965

YUAN Ying, SHEN Wei-xing, ZHANG Pei-yun, JIN Shu-yi, ZHU Li. Effects of EGb on apoptosis and NOS activity in rat hippocampal neurons induced by hypoxia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(9): 996-1000.

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