Inhibition of prodigiosin on migration and invasion of cancer cells and expression of MMPs

Abstract

Abstract: AIM: To observe the inhibition and mechanisms of prodigiosin on migration and invasion of cancer cells in vitro.METHODS: The migration and invasion of cells was measured by wound healing assay and the transwell assay. The expression of MMP-2,-9 was analyzed by gel zymography assay. The expression of NF-κB protein in cytoplasm was measured by western-blot. The concentration of prodigiosin in 95-D cells was analyzed by HPLC.RESULTS: The inhibitive effects on migration of the cells reached 42 %when cells were treated with 2. 5 μmol·L^-1 prodigiosin for 12 h and under the concentration of no cytotoxicity actions. The invasion of 95-D cells was dose-dependently inhibited by prodigiosin with EC50 of 5 μmol·L^-1.In addition, prodigiosin effectively inhibited the activity of MMP-2,-9. Prodigiosin also elevated the protein level of NF-κB in cytoplasm and then inhibited the activity of NF-κB. The results of HPLC showed that prodigiosin effectively entered cells, and the pharmacological effect of prodigiosin was dependent on the drug concentration in cells.CONCLUSION: Prodigiosin can effectively enter cells and inhibit the expression of MMP-2,-9 through the inhibition of the activity of the NF-κB, thereby it can inhibit the invasion and metastasis of tumor cells.

Key words: invasion, metastasis, MMP-2,-9, NF-κB, prodigiosin

CLC Number:

R979.1
R966

ZHANG Jing, SHEN Ya-Ling, LIU Jian-Wen. Inhibition of prodigiosin on migration and invasion of cancer cells and expression of MMPs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1091-1095.

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