Abstract: Aim To investigate the relationship between polypo rus polysaccharide (PUPS) and changes of int racellular calcium in human T24 bladder cancer cells. Methods Human T24 cells were cultured with PUPS and intracellular calcium level was measured with the fluorescent Ca²⁺ indicato r calcium crimson- AM and the laser scanning confocal microscopy.Results After human T24 cells were treated with a low dose of PUPS (0.2 mg·L^-1), the int racellular calcium was decreased; while with PUPS (> 1.0 mg·L^-1), the int racellular calcium became increased, and kept on ahigher level.Conclusion PUPS participates in the process of calcium message transduct ion.

Key words: polyporus polysaccharide, T24 bladder cancer cell, Ca²⁺