Effect of ketamine on synaptic long-term potentiation in hippocampus in rats with neuropathic pain

Abstract

Abstract: AIM: To investigate the effect of ketamine on synaptic long-term potentiation (LTP) in the hippocampus in rats with neuropathic pain.METHODS: Eighteen adult male Wistar rats weighing 190-220 g were randomly divided into three groups (n=6): model of neuropathic pain group (model group),sham operating group, and ketamine group.Neuropathic pain was produced by ligation of the left L_4,5 spinal nerve.The behaviour of the rats was evaluated at 1, 2 and 3 week after surgery.The pain threshold was measured before surgery,at 1, 2 and 3 week after surgery.The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was measured at 3 d after the last measurement of pain threshold.LTP was induced by high-frequency stimulation(HFS).Ketamine(25 mg/kg i.p.)was injected at 20 min before HFS.RESULTS:Compared with the sham operating group,the pain threshold was significantly lower in the model group and ketamine group.Compared with the model group,the amplitude of LTP was significantly lower in the ketamine group (P<0.01).CONCLUSION: Ketamine might inhibit the facilitation of synaptic LTP induced by neuropathic pain in the CA1 area of the hippocampus.

Key words: Neuralgia, Long-term potentiation, Hippocampus, Ketamine

CLC Number:

R441.1

WEI Hui-ming, MA Wei-qing, YANG Yun-li, DONG Fa-tuan, ZHANG Chen-hua, WANG Hui-ming. Effect of ketamine on synaptic long-term potentiation in hippocampus in rats with neuropathic pain[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 170-173.

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