Comparative study on enriching blood effect of Fo-shou-san with different ethanol extraction

Abstract

Abstract: AIM: To study the enriching blood effect of Fo-shou-san (FSS) extracted with different ethanol concentration on blood deficiency animal and approach the action characteristics of enriching blood.METHODS: The blood deficient mice model was induced by injection of phenylhydrazine and cyclophosphamide. The body weight of mice was recorded during the induction of model. After the model was established, blood was collected from the retroorbital plexus, and the blood routine, thymus index, spleen index, and liver index were determined.Total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in liver were detected by colorimetric method and xanthine oxidase method, respectively.Those indexes were calculated to a single index by the weight coefficient method for exploring the total enriching blood effect.RESULTS: FSS extracted with different ethanol concentration could increase the number of white blood cell, red blood cell, platelet and hematocrit in model mice; increase weight, thymus index, and spleen index of model mice, while decrease liver index of model mice; and increase the activity of T-AOC and SOD in liver. The order of FSS extracted with different concentration of ethanol on total enriching blood effect was: 40%>30%>20%>50%>15%>70%>10%>5%>95%>0%.CONCLUSION: FSS extracted with different ethanol concentration has the activity of enriching blood on blood deficiency model mice, and 40% ethanol extraction showed the best effect.

Key words: Fo-shou-san, Angelica sinensis(Oliv.)Diels, Ligusticum chuanxiong Hort, Blood deficiency, Enriching blood

CLC Number:

R965.2

HUANG Mei-yan, LI Wei-xia, TANG Yu-ping, GUO Jian-ming, SHANG Er-xin, LIU Xin, LIU Li, DUAN Jin-ao. Comparative study on enriching blood effect of Fo-shou-san with different ethanol extraction[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(8): 841-846.

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