Protective effects of recombinant human interleukin 10 on lung ischemia-reperfusion injury in rats

Abstract

Abstract: AIM: To evaluate the protective effects of recombinant human interleukin 10 on lung ischemia-reperfusion injury in rats and its possible mechanisms.METHODS: Rat models of lung ischemia-reperfusion were established by clamping of the left pulmonary arteries,left pulmonary veins and left main bronchus.72 healthy Wistar rats were randomly divided into three groups: surgical control group (group Control),ischemia-reperfusion group(group IR) and recombinant human interleukin 10 pretreated group(group rhIL-10). Every group included 24 rats (8 rats at 45 min ischemia,60 min and 120 min reperfusion respectively).Rats were sacrificed at relative time point collecting the plasma and lung sample. The plasma contents of malondialdehyde (MDA) and TNF-α were determined. Lung pathology were examined.RESULTS: The plasma content of MDA was not different statistically among three groups after 45 min of ischemia (P＞0.05).Both group IR and group rhIL-10 showed significantly increased MDA content relative to group Control, while group rhIL-10 was lower than that of group IR after 60 min of reperfusion and 120 min of reperfusion (P＜0.05). The plasma content of TNF-α in group IR was increasing significantly relative to group Control after 45 min of ischemia (P＜0.05). The content of TNF-α in group rhIL-10 was not different statistically compared with group Control (P＞0.05). Both group IR and group rhIL-10 showed significantly increased TNF-α content relative to group Control while group rhIL-10 was lower than that of group IR after 60 min of reperfusion and 120 min of reperfusion (P＜0.05). Pathological examination showed severe polymorphonuclear leukocytes infiltration with bleeding. Group IR was worse than group rhIL-10 on the pathological examination.CONCLUSION: Pretreatment with rhIL-10 protected the lungs against ischemia-reperfusion injury possibly by suppressing PMN infiltration,reducing production of oxygen radicals,inhibiting reacting of TNF-α.

Key words: rhIL-10, Lung ischemia-reperfusion injury, Oxygen radicals

CLC Number:

R965.2

SI Yu-long, ZHANG Wen-kai. Protective effects of recombinant human interleukin 10 on lung ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(11): 1239-1243.

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