Effects of tectorigenin on the secretion of extracellular matrix in Hepatic stellate cells

Abstract

Abstract: To investigate the effect of tectorigenin on the secretion of extracellular matrix (ECM) in Hepatic stellate cells.METHODS: HSC-T6 cells were incubated with tectorigenin at different concentrations, the mRNA expression of α-SMA, pro-collagen-α₁(Ⅰ), MMP-13 and TIMP-1 were detected by real-time PCR.RESULTS: Tectorigenin significantly decreased the expression of α-SMA in HSC-T6, as well as the mRNA expression of pro-collagen-α₁, and tectorigenin significantly elevated the mRNA expression of MMP-13, while decreasing the mRNA expression of TIMP-1.CONCLUSION: Tectorigenin could reduce the synthesis and secretion of type Ⅰ collagen in HSC, thereby reducing the deposition of extracellular matrix; the other hand, tectorigenin could promote the degradation of extracellular matrix, so as to have anti-fibrosis potentials.

Key words: Tectorigenin, Liver fibrosis, Hepatic stellate cells, Extracellular matrix

CLC Number:

R965.2

WANG Zhi-yong, WU Jun-hua, WANG Yu-rong, YU De-cai, DING Yi-tao. Effects of tectorigenin on the secretion of extracellular matrix in Hepatic stellate cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(2): 175-180.

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