Effect of Wedelolactone on COX-2, NO and TNF-α expression in lipopolysaccharide induced RAW264.7 cells

Abstract

Abstract: AIM: To study effect of Wedelolactone on COX-2, NO and TNF-α expression in lipopolysaccharide (LPS) induced RAW264.7 cells.METHODS: The contents of PGE₂, TNF-α, NO were detected by ELISA methods, expression of COX-2 protein in RAW264.7 cells was measured by MTT assay, in the presence or absence of 10 μg/mL lipopolysaccharide (LPS) or Wedelolactone (0.2, 2, 20 μmol/L).RESULTS: The expression of COX-2 protein was significantly induced by LPS, Wedelolactone inhibited the expression of COX-2 protein significantly elicited by LPS in RAW264.7 cells; LPS-induced PGE₂, TNF-α, NO was significantly inhibited by Wedelolactone in RAW264.7 cells, in dose dependent manners.CONCLUSION: The anti-inflammatory mechanisms of TGA may suppress production of PGE₂ through inhibiting COX-2 gene expression, and inhibit activity of NO and TNF-α.

Key words: Wedelolactone, Lipopolysaccharide, Cell culture

CLC Number:

R965.2

CHEN Jie, LI Rui-ming, XU Jing, GUAN Bi-qiong, LUO Zi-ling. Effect of Wedelolactone on COX-2, NO and TNF-α expression in lipopolysaccharide induced RAW264.7 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(2): 171-174.

References

[1] Kobori M, Yang Z, Gong D, et al. Wedelolactone suppresses LPS-induced caspase-11 expression by directly inhibiting the IKK complex[J]. Cell Death Differ, 2004, 11(1):123-130.
[2] Melo PA, do Nascimento MC, Mors WB, et al. Inhibition of the myotoxic and hemorrhagic activities of crotalid venoms by Eclipta prostrata (Asteraceae) extracts and constituents[J]. Toxicon, 1994, 32(5): 595-603.
[3] Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica[J]. Phytomedicine, 2004, 11(4): 361-365.
[4] Wagner H, Geyer B, Kiso Y, et al. Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea[J]. Planta Med, 1986, 5(1): 370-374.
[5] 严梅娣, 叶孟, 林蕾, 等. 槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响[J]. 中国临床药理学与治疗学, 2010(2): 126-129.
[6] 周国勇, 胡森, 吕艺, 等. 卡巴胆碱对内毒素刺激大鼠腹腔巨噬细胞炎症细胞因子表达的影响[J]. 中国临床药理学与治疗学, 2009(12): 1361-1365.
[7] Ho FM, Lai CC, Huang LJ, et al. The anti-inflammatory carbazole, LCY-2-CHO, inhibits lipopolysaccharide-induced inflammatory mediator expression through inhibition of the p38 mitogen-activated protein kinase signaling pathway in macrophages[J]. Br J Pharmacology, 2004, 141(6): 1037-1047.
[8] Lee AK, Sung SH, Kim YC, et al. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κBα phosphorylation, C/EBP and AP-1 activation[J]. Br J Pharmacol, 2003, 139(1): 11-20.
[9] Takayukl N, Norlko M, Glovanna T, et al. Prostaglandin E2 promotes degranulation-independent release of MCP-1 from mast cells[J]. J Leukoc Biol, 2006, 79(1):1-10.
[10] Xiao C, Li J, Dong X, et al. Anti-oxidative and TNF-alpha suppressive activities of puerarin derivative (4AC) in RAW264.7 cells and collagen-induced arthritic rats[J]. Eur J Pharmacol, 2011, 666(1/2/3): 242-250.

[1]	ZHANG Lei, XIAO Xingpeng, DING Huang . Silencing ZKSCAN3 gene expression aggravates lung injury induced by LPS in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 164-170.
[2]	TIAN Dahao, LU Linming, ZHI Hui, ZHOU Jue, WANG Xiaoqing, JIANG Yuhua. AHVAC-I inhibits the proliferation in human primary gastric cancer cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 505-511.
[3]	FU Xiaomei, HUO Ran, DENG Sai, LIN Chaojin, FAN Jinghao, WANG Ping, LI Songpei, QIN Aiping, ZOU Minghui, YU Xiyong. Exosomes derived from LPS-stimulated bone marrow mesenchymal stem cell improve myocardial inflammation and fibrosis after myocardial infarction in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(8): 841-851.
[4]	LIU Huanmiao, CHEN Zhuohui, WU Di, HUANG Xueting, WAN Qiquan. Insulin attenuates the inhibition effect of lipopolysaccharide on Na+-K+-ATPase α1 via PI3K/AKT and PI3K/ERK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(8): 896-902.
[5]	CHEN Shaozhong, LIN Meise, CHENG Limin, ZHU Lele. Effects of salidroside injection on pulmonary vascular permeability in rats with septic shock induced lung injury induced by lipopolysaccharide [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1256-1262.
[6]	CAO Yingya, CHEN Qun, WANG Zhen, WU Jingyi, JIANG Xiaogan, JIN Xiaoju, LU Weihua. Role of macrophages in LPS-induced apoptosis of intestinal epithelial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1142-1146.
[7]	ZHENG Shuang, FU Qinlei, ZHOU Lingping, PENG Chuanpeng, XU Honglei. Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 148-153.
[8]	ZHONG Zhengling，ZHANG Fei，JIANG Yuhua，ZHANG Weiting，TONG Jiucui，LI Juan，CHU Jiru，XIE Haitang. Protective effects of Ferulic acid on acute liver injury induced by D-GalN/LPS in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(12): 1335-1339.
[9]	WANG Di, ZHANG Huanhuan, FANG Jie, ZHONG Yushen, YU Chenhuan. Effects of limoninon on LPS-induced acute lung injury in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 8-12.
[10]	LIU Xiaolin, YU Meiling, XIE Jing, ZHANG Lina, XU Wenfang, YE Ming, LI Qianqian, CHEN Yuhua. Study on the relationship between constipation, colonic mucosal inflammation, intestinal LPS and Parkinson's disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 456-460.
[11]	GE Bobo，QU Hongdang，CHEN Yuhua，HU Caicai，LI Li，JIANG Mujun，XU Juanjuan. LPS-induced chronic Parkinson's disease in mice α-synuclein in the emergence and metastasis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 241-248.
[12]	CHEN Yan, YE Bin, MOU Wen-yuan, YU Cheng-bo, LIN Bu, YIN Xiang-peng, XU Hong-miao. Effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 493-498.
[13]	MO Hong-ying, JIANG Yong-nan, LI Yi-min, LAI Le, XIAO Zheng-lun. Effect of MIF siRNA on the expression of Toll-like receptors and down stream signaling components in A549 cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 382-387.
[14]	ZHU Hai-long, ZHANG Gen-bao, LI Wei, ZHANG Cui. Protective effects of ERK1/2 inhibitor PD98059 on multiple organ dysfunction in disseminated- intravascular-coagulation rat [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(2): 159-163.
[15]	YU Meng-chen, PAN Xi-chun, LI Xiao-li, LI Jun, XIAO Kang-kang, LI Bin, ZHOU Hong. Artesunate enhance the ability of peritoneal macrophages endocytosing LPS and Escherichia coli and its mechnisms [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 40-46.