Effect of cyclooxygenase 2 inhibitor on ventilator-induced lung injury in rats

Abstract

Abstract: AIM: To investigate the effect of cyclooxygenase 2 inhibitor on ventilator-induced lung injury in rats. METHODS: Thirty healthy adult male SD rats weighing 300-350 g were randomly divided into 3 groups (n=10 each): group TV (traditional tidal volume VT = 8 mL/kg); group HV(high tidal volume VT=40 mL/kg); group HV+NS398(pretreated with NS398 8 mg/kg before ventilating). The animals were anesthetized with intraperitoneal 20% urethane 8 mL/kg, tracheotomized, intubated and mechanically ventilated for 4 h. Group HV+NS398 was given with intraperitoneal injection at 30 min before mechanical ventilation. The animals were sacrificed at 4 h after mechanical ventilation. The lungs were removed for determination of bronchoalveolar lavage fluid (BALF) TNF-α, IL-6, TXB₂, total protein concentration, wet to dry weight ratio (W/D). Expression of aquaporin 1(AQP1) was analyzed by Western Blot. RESULTS: Expression of AQP1 was decreased significantly in group HV than that in group TV(P＜0.05). The BALF concentration of TNF-α, IL-6, TXB₂, total protein and W/D were higher in group HV than those in group TV(P＜0.05). Cyclooxygenase-2 inhibitor significantly attenuated the HV-induced changes listed above in group HV+NS398. CONCLUSION: Cyclooxygenase 2 inhibitor attenuates ventilation-induced lung injury in rats. Its mechanism might relate to upregulate expressionof aquaporin 1and lessen inflaming response.

Key words: Cyclooxygenase 2 inhibitor, Respiration, artificial, Respiratory distress syndrome,Adult, Aquaporin

CLC Number:

R614

JIN Li-da,WANG Liang-rong, XIONG Xiang-qing, LIN Li-na, SHAN Yuan-lu, ZHOU Jun-hui. Effect of cyclooxygenase 2 inhibitor on ventilator-induced lung injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(7): 732-735.

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