The changes of NF-κB promote rheumatoid arthritis fibroblast-like synoviocytes apoptosis by Danshen injection

Abstract

Abstract: AIM: To investigate the changes of expressions and the location of P65 and P50 in rheumatoid arthritis fibroblast-like synoviocytes (FLSs) by Danshen injection.METHODS: Fibroblast-like synovial cells obtained from synovial fluid by primary culture, and then treated with final concentrations of 0, 0.2 and 0.4 mg/mL of Danshen injection for 24 h. The expressions and location of P65 and P50 were detected with immunocytochemistry and Western blot.RESULTS: Immunocytochemistry showed that in the absence of drugs under the conditions of stimulation, either P65 or P50 was mainly concentrated in the cytoplasm, when the final concentration of Danshen injection reaches 0.2 mg/mL, both of them had a tendency to nuclear transfer, when the final concentration of Danshen injection to 0.4 mg/mL, both of them significantly accumulated in the nucleus. Western blot results showed that the expressions of P65 and P50 in the nucleus increased with the elevated concentration of Danshen injection, and when the concentration of Danshen injection reach to 0.4 mg/mL, the expressions of both of them increased significantly (P<0.05, n=4).CONCLUSION: Danshen injection can adjust rheumatoid arthritis fibroblast-like synoviocytes P65 and P50 from the cytoplasm to the cell nuclear transfer, and thus promote apoptosis of fibroblast-like synoviocytes.

Key words: Danshen injection, Rheumatoid arthritis, Fibroblast-like synoviocytes, NF-κB

CLC Number:

R593.22

LIU Qing-song, ZHU Xing-chun, JIANG Hong, CAI Yan, YUAN Guo-hua, YANG Ming-hui, ZHANG Jun, LI Jun-an, TANG Zhong. The changes of NF-κB promote rheumatoid arthritis fibroblast-like synoviocytes apoptosis by Danshen injection[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(8): 846-850.

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