Inhibitory effect of colon-targeted ball of scorpion venom on young rats with chronic visceral hypersensitivity

Abstract

Abstract: AIM: To explore the inhibitory effect of colon- targeted ball of scorpion venom on model young rats with chronic visceral hypersensitivity. METHODS: Neonatal male Sprague-Dawley(SD)rats were used as study subject. Model rats were received 60 mm Hg colon irritation (CI) for a minute once daily from post neonatal days 8 to 14; control rats were received the same processes except CI. The experiment groups were divided into control group , control blank ball group , control scorpion venom ball group, model group, model blank ball group, model scorpion venom ball group ( low ), model scorpion venom ball group ( medium ) and model scorpion venom ball group ( high ). Colon- targeted ball of scorpion venom was given by oral on the twenty-eighth day, one time a day, in total 14 days. The spike of external oblique muscle of abdomen(EOMA)were examined to assess the visceral sensitivity of rats; The field potential LTP was observed in control and model young rats by the recording of field potential in hippocampal CA1 region in vitro. RESULTS: The spike amplitude of EOMA was significantly stronger in model young rats than those in control young rats, the spike amplitudes of EOMA were decreased in model rats by oral colon- targeted ball of scorpion venom (P<0.05). The spike amplitudes of LTP was significantly stronger in model young rats than those in control young rats (P<0.05). Compared with model young rats, the spike amplitudes of LTP were significantly decreased by oral colon- targeted ball of scorpion venom (P<0.05).However, there was no significant effect of colon- targeted ball of scorpion venom on control young rats (P>0.05). CONCLUSION: Chronic visceral hypersensitivity could be inhibited by Oral colon- targeted ball of scorpion venom in model young rats.

Key words: Colon- targeted ball of scorpion venom, Long-term potentiation(LTP), Hippocampus, Spike of external oblique muscle of abdomen

CLC Number:

R965.2

LIN Guo-wei, LIN Chun, YE Rong, ZHENG Wei, HUANG Ren-jie, Lin Zhi-ming. Inhibitory effect of colon-targeted ball of scorpion venom on young rats with chronic visceral hypersensitivity[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1130-1136.

References

[1] 张瑞德,王欢,马晓玲,等.辽宁产东亚钳蝎毒的镇痛作用[J].中国医科大学学报,1997,26(2):140-143.
[2] 谢兴亮,杨明,邱雪兰,等.pH-时滞型愈肠宁结肠靶向片的制备及体外释放评价[J].中国中药杂志,2007,32(13):1280-1284.
[3] 杨明,谢兴亮,马鸿雁,等.pH依赖型愈肠宁结肠靶向片的制备及体内外释放评价[J].中草药,2006,37(5):680-684.
[4] Al-Chaer ED, Kawasaki M, Pasricha PJ.A new model of chronic visceral hypersensitivity in adu1t rats induces by colon irritation during postnata1 development[J] . Gastroenterology,2000, 119(5) :1276-1285.
[5] 蔡琴燕,唐影,黄扬,等.动物麻醉机在肠易激综合征模型大鼠评价中的应用[J].福建医科大学学报, 2011,45(1):29-33 .
[6] 祝福存,陈瑜,林春,等.尼氟酸抑制慢性内脏痛大鼠海马CA1区突触长时程增强[J].神经解剖学杂志, 2012,28(2):151-156.
[7] 邓艳萍,徐国柱,王伟,等.蝎毒注射液镇痛效果及安全性临床评价[J].中国药学杂志, 2002,37(6):459-462.
[8] 沈小珩,许建中,朱伟嵘,等.蝎毒注射液治疗癌痛[J].中国癌症杂志, 2001, 11(3) : 200-204.
[9] 刘敏芝,刘洪臣,黄民,等.东亚钳蝎毒的镇痛作用与吗啡镇痛作用的比较[J] .白求恩医科大学学报,1995,21(3):260-261.
[10] Bennett MR.The concept of long term potentiation of transmission at synapses[J]. Prog Neurobiol ,2000, 60(2): 109-137.
[11] 王成裕,雷留根,韩雪飞,等.蝎毒素IV尾静脉注入后的镇痛作用[J] .河南医科大学学报,1996,31(3) :8-12.
[12] 张瑞德,李新新,马晓玲,等.辽宁产东亚钳蝎毒镇痛作用与脑内中枢关系的研究[J] .中国医科大学学报,1997,26(3):230-232.
[13] 李宁,吕欣然,韩惠蓉,等.蝎毒与吗啡中枢镇痛作用效果比较[J].中草药, 1998,29(11) :750-752.
[14] 雷留根,王成裕,韩雪飞,等.蝎毒素IV的中枢镇痛作用[J].河南医科大学学报,1996,31(1) : 31-34.
[15] Bliss TV, Lomo T.Long-lasting potentiation of synaptic transmission in the dentate area of the unanaesthetized rabbit following stimulation of the perforant path[J].Physiology, 1973,232(2): 331-356.
[16] Ji RR, Woolf CJ.Neuronal plasticity and signal transduction in nociceptive neurons: implications for the initiation and maintenance of pathological pain[J]. Neurobiol Dis ,2001, 8(1): 1-10.
[17] Woolf CJ, Mannion RJ.Neuropathic pain: aetiology, symptoms,mechanisms, and managements[J]. Lancet,1999, 353: 1959-1964.
[18] Bird CM, Burgess N.The hippocampus and memory: insights from spatial processing[J]. Nat Rev Neurosci, 2008, 9: 182-194.
[19] 魏辉明,麻伟青,李治贵,等.神经病理性疼痛老年大鼠海马CA1区突触长时程增强的变化[J]. 临床麻醉学杂志,2008, 24(12):1056-1059.
[20] 魏辉明,杨云丽,麻伟青,等.丙泊酚对大鼠海马CA1区长时程增强的影响[J].中国临床药理学与治疗学,2007,12(11):1264-1268.
[21] 邢国刚,刘风雨,姚磊,等.神经病理痛大鼠脊髓背角突触传递的长时程可塑性变化[J].北京大学学报,2003,35(3):226-230.
[22] 马骋,李翠贤,易建良,等.电针对大鼠神经病理性疼痛模型DRG和脊髓Glu和Asp含量的影响[J].针刺研究,2008,33(4): 250-254.